Effects of punicalagin on intestinal microbiota and inflammatory factors in mice with ulcerative colitis
Objective To observe the impact of punicalagin on intestinal microbiota and inflammatory factors in mice with ulcerative colitis(UC).Methods The mice were divided into four groups,namely control group(Con),UC model group(UC),5-aminosalicylic acid treatment group(5-ASA),and punicalagin administration group(Pun),5 mice in each group.After administered punicalagin,the body weight,stool blood,and stool shape of the mice were observed,and the disease activity index(DAI)scores were determined.Hematoxylin-eosin(HE)staining was used to observe colon tissue sections for morphological analysis,pathological scoring,and statistical valuation.Serum levels of IL-6,TNF-α,IL-1β,IL-8 and IL-10 were measured using ELISA.Intestinal microbiota of the mice were analyzed through 16S rRNA high-throughput sequencing.Results Compared to the UC model group,the Pun group exhibited a significant decrease in DAI score.HE results indicated that punicalagin improved colon injury by restoring intestinal glands and crypt structure while reducing inflammatory cell infiltration in UC mice.Serum levels of proinflammatory cytokines IL-6,IL-8,IL-1β and TNF-α significantly decreased in the Pun group(P＜0.01),whereas that of anti-inflammatory cytokine IL-10 significantly in-creased(P＜0.001).Analysis of sequencing data revealed that punicalagin enhanced intestinal microbiota diversity while increasing relative abundances of Prevotellaceae_UCG-001 and Lactobacillus but decreasing those of Akkermansia and Li-gilactobacillus.Conclusion Punicalagin can effectively alleviate the symptoms of UC in mice,and achieve the therapeut-ic effect by reducing serum inflammatory factor levels and regulating intestinal microbiota balance.This provides new ideas for the clinical treatment of UC.
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