α-1,6葡聚糖与低聚异麦芽糖对益生菌和人体肠道菌群的影响比较研究
Comparative study on the effects of α-1,6-glucan and oligomeric isomaltose on probiotics and human gut microbiota
杨玉洁 1吴迪楠 2李宇轩 3何莹 3胡晓飞 3孙雯颖 4李彧媛 2李明 2鞠伟伟5
作者信息
- 1. 安徽医科大学第一附属医院,安徽合肥 230022;大连医科大学
- 2. 大连医科大学
- 3. 广州华汇生物实业有限公司
- 4. 大连市中心医院
- 5. 大连医科大学附属第二医院
- 折叠
摘要
目的 比较α-1,6葡聚糖和低聚异麦芽糖对益生菌的体外增殖作用,评价2种低聚糖对人体肠道菌群的影响.方法 脆弱拟杆菌在分别含有2种低聚糖的不同培养基中发酵,活菌计数绘制生长曲线;采集12位健康志愿者的新鲜粪便,将肠道菌群悬液按10％的接种量分别接种于Control组(含氮基础培养基)、α-1,6葡聚糖组(含氮基础培养基加1％α-1,6葡聚糖)及低聚异麦芽糖组(含氮基础培养基加1％低聚异麦芽糖),37℃恒温静态厌氧发酵24 h.采用高通量测序技术分析粪便发酵液菌群结构;应用高效液相色谱法检测粪便发酵液中短链脂肪酸(SCFAs)水平.结果 α-1,6葡聚糖促进脆弱拟杆菌增殖的能力较低聚异麦芽糖强[(5.27±0.33)vs(4.80±0.66),×108 CFU/mL];粪便发酵实验显示α-1,6葡聚糖可降低粪便中厚壁菌门/拟杆菌门比值(t=2.821,P=0.0478),显著提升 SCFAs 水平(丙酸:t=3.750,P=0.0092;丁酸:t=5.747,P=0.0018).结论 α-1,6葡聚糖可以改善肠道菌群结构并促进SCFAs产生.
Abstract
Objective To compare the effects of α-1,6-glucan and oligomeric isomaltose on probiotics and human gut mi-crobiota.Methods Bacteroides fragile ferments in different media containing two types of oligosaccharides,and the growth curve was plotted by counting the live bacteria;Fresh feces from 12 healthy volunteers were collected,and the sus-pension of gut microbiota were inoculated to the control group(nitrogen-containing basic culture medium),α-1,6-glucan group(nitrogen-containing basic culture medium with 1％α-1,6-glucan),and oligosaccharide isomaltose group(nitrogen-containing base medium with 1％oligosaccharide isomaltose)at a 10％inoculation rate.The static anaerobic fermentation was carried out for 24 hours under constant temperature conditions at 37 ℃.High-throughput sequencing technology was used to analyze the microbial community structure of fecal fermentation broth;Short chain fatty acids(SCFAs)levels in fecal fermentation broth were detected by High Performance Liquid Chromatography(HPLC).Results α-1,6-glucan had a stronger ability to promote the proliferation of Bacteroides fragile compared to oligomeric isomaltose[(5.27±0.33)vs(4.80±0.66),×108 CFU/mL];Reduce the ratio of Firmicutes/Bacteroidetes in feces(t=2.821,P=0.047 8)and significantly increase the content of SCFAs(Propionate:t=3.750,P=0.009 2;Butyrate:t=5.747,P=0.001 8).Conclusion α-1,6-glucan can improve the structure of gut microbiota and promote the production of SCFAs,which may alleviate intestinal inflam-mation and alleviate obesity.
关键词
α-1,6葡聚糖/低聚异麦芽糖/肠道菌群/短链脂肪酸Key words
α-1,6-glucan/Oligomeric isomaltose/Gut microbiota/Short chain fatty acids引用本文复制引用
出版年
2024
NETL
NSTL
万方数据