Characteristics of intestinal flora in patients with major depressive disorder and its correlation with inflammatory factors
Objective To explore the characteristics of intestinal flora in patients with major depressive disorder(MDD),and analyze its correlation with inflammatory factors and the score of the Hamilton depression scale-24(HAMD-24).Methods A total of 58 treatment-naïve first-episode MDD patients who met the inclusion criteria and 50 healthy gender-and age-matched controls(HCs)were included.HAMD-24 was used to assess the severity of depressive symptoms,and 16S rRNA gene sequencing was used to assess the composition of intestinal flora.Enzyme linked immunosorbent assay was used to detect C-reactive protein(CRP),interleukin-1β,interleukin-6 and tumor necrosis factor-α in peripheral blood.Spearman correlation analysis was used to evaluate the correlation of intestinal flora with inflammatory factors and HAMD-24 score.Results The CRP level in MDD group was significantly higher than that in HC group(t=8.353,P＜0.050).Al-pha diversity analyses(Shannon index,Simpson index,Pielou index)showed that there were significant differences in in-testinal flora between MDD group and HC group(H=8.529,H=8.893,H=10.825,all P＜0.050),and beta diversity ana-lyses showed that the difference in the distribution of intestinal flora between the two groups was statistically significant(F=3.997,P＜0.050).Further linear discriminant analysis Effect Size analysis found that,at the genus level,the relative abundances of Bifidobacterium,Blautia,Coprococcus,Megamonas and Dorea were higher in MDD group,while those of Bacteroides,Faecalibacterium,Roseburia and Dialister were higher in HC group.Spearman correlation analysis showed that Blautia and Coprococcus were positively correlated with CRP and HAMD-24 score(r=0.258,r=0.284,r=0.235,r=0.262,P＜0.050),while Faecalibacterium and Roseburia were negatively correlated with CRP(r=-2.030,r=-0.221,all P＜0.050).Conclusion MDD patients have altered intestinal flora and increased plasma CRP level,and the altered intest-inal flora is closely related to plasma CRP level and HAMD-24 score.
Major depressive disorderIntestinal floraInflammatory factor
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