Effect of Dendrobium huoshanense on the diversity of gut microbiota in mice with diarrhea induced irritable bowel syndrome
Objective To observe the effects of Dendrobium huoshanense on the gut microbiota of mice with diarrhea predominant-irritable bowel syndrome(IBS-D),and provide a basis for the study of the therapeutic mechanism of Dendrobium huoshanense on IBS-D.Methods Twenty-four SPF grade male mice were randomly divided into normal control group,model group,Dendrobium huoshanense group,and pinaverium bromide group,with 6 mice in each group.The IBS-D model was prepared by gavage of senna leaves combined with restrained tail clamping stress.After successful modeling,the Dendrobium huoshanense group and pinaverium bromide group were given corresponding drug doses by gavage for one week.The general status,fecal characteristics,fecal moisture content,abdominal withdrawal reflex score were compared among groups;changes in gut microbiota species and abundance of mice in each group were detected us-ing 16S rRNA gene high-throughput sequencing.Results Compared with the normal controls,the IBS-D model mice showed poor mental health,increased bowel movements,increased fecal moisture content and visceral sensitivity,and de-creased diversity and abundance of gut microbiota.After intervention with Dendrobium huoshanense,compared with the model controls,the mental status of mice in the Dendrobium huoshanense group was improved,and the fecal characterist-ics returned to normal,while the fecal moisture content and visceral sensitivity decreased.The relative abundance and com-position of species indicated that Dendrobium huoshanense increased the diversity of gut microbiota in IBS-D mice and significantly increased the relative abundances of beneficial bacteria genera such as norank_Muribaculaceae,Lactobacillus,Alloprevotella,and decreased those of pathogenic bacteria such as Escherichia and Shigella in the Proteobacteria phylum.Conclusion Dendrobium huoshanense reduce visceral hypersensitivity in IBS-D mice,possibly by restoring the diversity of gut microbiota in IBS-D mice,regulating the relative abundance of gut microbiota,and exerting therapeutic effects on IBS-D.
万方数据
维普
