Effects of fecal microbiota fluid from individuals with type 2 diabetes on intestinal flora of different diets mice and its correlation with glycolipid metabolism and inflammation
Objective To explore the effect of type 2 diabetes(T2DM)fecal bacteria on intestinal flora of normal and high-fat diet mice,and analyze the correlation of intestinal flora with blood glucose,lipid and inflammation.Methods Forty male C57BL/6 mice were randomly divided into four groups:the normal diet(ND)group,the microbiota transplantation(MT)group,the high-fat diet(HFD)group,and the HFD+MT group,with 10 mice in each group.These mice underwent an 8-week intervention with human fecal microbiota from T2DM patients.The copies of Bacteroides thet-aiotaomicron,Parabacteroides distasonis,Akkermansia muciniphila,Faecalibacterium prausnitzii,Lactobacillus acido-philus and Escherichia coli in fecal samples were measured by RT-qPCR.The correlations between these species and post-prandial glucose(PPG),fasting blood glucose(FBG),total cholesterol(TC),triglycerides(TG)and inflammatory indices were analyzed.Results Significant differences in the relative abundances of E.coli,F.prausnitzii and L.acidophilus were observed among the four groups(F=8.081,P＜001;F=17.353,P＜001;F=7.181,P＜001).Compared with the ND group,the MT group,HFD group and HFD+MT group showed increased level of E.coli and decreased levels of F.praus-nitzii and L.acidophilus.Comparing with the HFD group and the HFD+MT group,the latter exhibited higher level of E.coli and lower level of F.prausnitzii.Notably,F.prausnitzii was significantly correlated with PPG,FBG,TC and LBP(r=-0.585,r=-0.639,r=-0.716,r=-0.626;all P＜0.05).E.coli demonstrated significant correlations with PPG,FBG,TC,TNF-α and LBP(r=0.455,r=0.545,r=0.456,r=0.451,r=0.433;all P＜0.05).Conversely,L.acidophilus showed signific-ant negative correlations with FBG,TC,TNF-α and LBP(r=-0.583,r=-0.497,r=-0.565,r=-0.614;all P＜0.05).Conclusion Fecal microbiota from patients with T2DM may disrupt the balance of intestinal microbiota in mice with normal or high-fat diets by altering the levels of E.coli,F.prausnitzii and L.acidophilus,inducing inflammation and sub-sequently causing glucose and lipid metabolism disorders.
万方数据
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