Objective To explore the characteristics of gut microbiota in patients with major depressive disorder(MDD),and analyze the correlation of disordered gut microbiota with depressive symptoms and sleep symptoms,so as to provide theoretical support for the pathophysiological mechanism of MDD with sleep problems.Methods One hundred and nine patients with MDD and 96 healthy controls(HCs)were enrolled in this study.Hamilton Depression Scale(HAMD-17)and Pittsburgh Sleep Quality Index(PSQI)were used to evaluate the depressive symptoms and sleep quality of the two groups.Stool samples of the two groups were collected and analyzed with 16S rRNA sequencing.Spearman correlation analysis was used to analyze the correlation of gut microbiota with depressive symptoms and sleep symptoms.Results The HAMD score(t=33.040,P＜0.001)and PSQI score(t=21.421,P＜0.001)were significantly higher in the MDD group than in the HC group.Simpson's index and Pielou's evenness index differed between the two groups(Z=2.178,P=0.029;Z=2.258,P=0.024).The PCoA showed no differences in the flora between the two groups.LEfSe analysis showed that Bi-fidobacterium and Blautia were the predominant genera in the MDD group.Correlation analysis showed that HAMD total score,PSQI total score and some factor scores were positively correlated with Blautia and Bifidobacterium and negatively correlated with Bacteroides and Roseburia.Conclusion Gut microbiota is altered in patients with MDD,which correl-ates with depressive symptoms and sleep symptoms.
Major depressive disorderSleep qualityGut microbiota
维普
万方数据
