Gut microbiota characteristics,short-chain fatty acid contents and anti-inflammatory effect in male rugby players
Objective To observe the changes of plasma inflammatory cytokines,gut microbiota structure and fecal short-chain fatty acid content in male rugby players.Methods A total of 30 male rugby players were recruited as the observa-tion group,with 15 male college students not majoring in physical education as the control group.Fasting venous blood and fecal samples were collected.The plasma levels of IL-1,IL-6,IL-10 and CRP were determined with ELISA.The composi-tion and structure of gut microbiota were compared using 16S rDNA sequencing within V3-V4 region.The contents of acetic acid,propionic acid,butyric acid and valeric acid in stool samples were determined by using gas chromatography-mass spectrometry(GC-MS).Results(1)The concentration of CRP in the plasma in observation group was significantly lower than that in the control group(P＜0.01).(2)Compared with the control group,the abundances of intestinal Bac-teroides vulgatus,Coprococcus comes and Roseburia inulinivorans in observation group were significantly higher(all P＜0.05).(3)Compared with the control group,the Observed species index and Shannon index of intestinal flora in obser-vation group were significantly higher than those in the control group(all P＜0.05).(4)Compared with the control group,the concentrations of acetic acid,propionic acid and butyric acid in the fecal samples in observation group were signific-antly increased(all P＜0.05).Conclusion The concentration of proinflammatory cytokine CRP in the plasma of rugby players decreased significantly,suggesting that sports training may play a role in anti-inflammatory effects.A large amount of exercise,direct physical confrontation and intense special training may have a profound impact on the gut microbiota system of rugby players.The change in the intestinal flora components increased the production of short-chain fatty acids.
万方数据
维普
