Status of vaginal microbiota in HIV-infected women receiving anti-retroviral therapy
Objective To learn the status of vaginal flora and its associated factors in HIV-infected Chinese women on standard anti-retroviral therapy(ART).Methods A total of 16 HIV-positive women in the infectious disease clinic of Beijing You'an Hospital and 11 healthy women in the gynecological clinic of Beijing Obstetrics and Gynecology Hospital were enrolled for questionnaire survey,and the vaginal secretions were collected for Illumina NovaSeq platform metagen-omic sequencing and microbiota analysis.Cervical secretions were collected for testing for sexually transmitted infections.Results(1)HIV-infected women generally had lower educational level(P=0.001)and misunderstandings about hygien-ic habits.They were used to vaginal douching(P＜0.001)and incorrect condom use(P=0.048).(2)The counts of peripher-al CD4+T cells varied among HIV(+)individuals despite receiving standard ART and having well-controlled viral loads.Diversity of vaginal microbiota in HIV(+)women was significantly higher,especially in women with CD4+T cells＜500/pL.Vaginal microbial diversity had no relation with HPV infection or ART duration.(3)Vaginal Lactobacillus were lower,while anaerobic bacteria such as Gardnerella,Prevotella,Atopotella,Streptococcus,Macrococcus,Anaerococcus and Corynebacterium were higher in HIV(+)women,especially those with CD4+T cells＜500/pL.(4)Actinomycetes,an opportunistic pathogen,was significantly higher in the vagina of HIV(+)menopausal women and those with lower CD4+T cell count in peripheral blood.Conclusion The degree of immune reconstitution and the count of peripheral CD4+T cells are closely related to the vaginal microecological health in HIV(+)women.Healthy intervention of vaginal microbiota is necessary.
Human immunodeficiency virusVaginal microbiotaImmune reconstitution
万方数据
维普
