一株口咽部链球菌E-24对铜绿假单胞菌诱导人呼吸道上皮细胞炎症因子的影响
Effect of an oropharyngeal streptococcal strain E-24 on inflammatory cytokines induced by Pseudomonas aeruginosa in human respiratory epithelial cells
孙冶 1李舒音 2车雨辰 3刘璇 4金晓东 4肖纯凌4
作者信息
- 1. 沈阳医学院病原生物学教研室,辽宁沈阳 110034;沈阳医学院辽宁省环境污染与微生态重点实验室,沈阳市肖纯凌劳模创新工作室
- 2. 沈阳医学院辽宁省环境污染与微生态重点实验室,沈阳市肖纯凌劳模创新工作室;沈阳医学院人事处
- 3. 沈阳医学院免疫学教研室
- 4. 沈阳医学院辽宁省环境污染与微生态重点实验室,沈阳市肖纯凌劳模创新工作室
- 折叠
摘要
目的 从健康儿童口咽部分离获得链球菌E-24,研究它对铜绿假单胞菌的抑菌作用以及抗炎症损伤的分子机制.方法 采用纸片分散法研究链球菌E-24抑制铜绿假单胞菌生长的有效成分.通过结晶紫染色法和平板计数法检测链球菌E-24对铜绿假单胞菌生物被膜形成能力和黏附能力的影响.通过透射电镜观察、免疫印记技术以及ELISA方法检测链球菌E-24逆转铜绿假单胞菌诱导肺炎的分子机制.结果 链球菌E-24发酵培养后,发酵液上清液是抑制铜绿假单胞菌的活性成分,而菌体没有抑菌活性.链球菌E-24上清液抑制铜绿假单胞菌生物被膜的形成和黏附.链球菌E-24上清液抑制铜绿假单胞菌诱导支气管上皮细胞BEAS-2B和16HBE铁死亡相关蛋白GPX4和SLC7A11的表达.链球菌E-24上清液抑制铜绿假单胞菌诱导BEAS-2B和16HBE细胞炎症因子TNF-α、IL-6及IL-8的表达.结论 口咽部分离培养的链球菌E-24作为潜在的微生态制剂,可抑制铜绿假单胞菌的生长和呼吸道细胞炎症损伤作用,对人体呼吸道健康起到保护作用.
Abstract
Objective To isolate Streptococcus E-24 from the oropharynx of healthy children and explore its antibacteri-al effect on Pseudomonas aeruginosa as well as the molecular mechanism of anti-inflammatory injury.Methods Paper dispersion method was employed to observe the effective components of Streptococcus E-24 in inhibiting the growth of Pseudomonas aeruginosa.Subsequently,crystal violet staining and plate counting were utilized to assess the biofilm form-ation and adhesion ability of Pseudomonas aeruginosa.Finally,transmission electron microscopy,Western Blot and ELISA were employed to elucidate the molecular mechanism by which Streptococcus E-24 reverses Pseudomonas aeru-ginosa-induced pneumonia.Results After the cultivation of Streptococcus E-24,the fermented supernatant was found to contain an active ingredient inhibiting Pseudomonas aeruginosa,while the bacterial cells had no bacteriostatic activity.The supernatant from Streptococcus E-24 inhibited biofilm formation and adherence of Pseudomonas aeruginosa.Furthermore,it also suppressed the expression of ferroptosis-related proteins GPX4 and SLC7A11 in bronchial epithelial cells BEAS-2B and 16HBE induced by Pseudomonas aeruginosa.Additionally,the supernatant from Streptococcus E-24 inhibited the in-duction of expressions of inflammatory factors TNF-α,IL-6 and IL-8 in BEAS-2B and 16HBE cells by Pseudomonas aeru-ginosa.Conclusion As a potential microecological agent,Streptococcus E-24 isolated from oropharynx can inhibit the growth of Pseudomonas aeruginosa and inflammatory damage of respiratory cells,and play a protective role in human res-piratory health.
关键词
呼吸道/链球菌E-24/铜绿假单胞菌/炎症损伤/铁死亡Key words
Respiratory tract/Streptococcus E-24/Pseudomonas aeruginosa/Inflammatory injury/Ferroptosis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据