摘要
目的 通过16SrRNA基因测序技术对食管鳞癌患者和健康人群的肠道微生物群进行分析,为该类患者的治疗提供参考.方法 按照纳入、排除标准收集2023年7月至2023年12月河南省肿瘤医院收治的42名食管鳞癌患者(EC组)和33名性别、年龄匹配的健康人(NC组)的新鲜粪便样本,并通过16S rRNA基因测序技术对肠道微生物群进行分析.结果 在门水平上,与NC组相比,EC组患者肠道放线菌门和变形菌门丰度显著升高.在属水平上,EC组患者肠道埃希-志贺菌属、肠球菌属、经黏液真杆菌属、克雷伯菌属、柯林斯菌属和双歧杆菌属丰度显著增加,而拟杆菌属、嗜血杆菌属、小杆菌属、韦荣球菌属、普雷沃菌属、巨单胞菌属和粪杆菌属显著减少.结论 采用16S rRNA基因测序技术有望筛选出肠道菌群与食管鳞癌发生发展的相关预警因子,从肠道微生物群变化方面预测食管鳞癌的发生发展.
Abstract
Objective To analyze the intestinal microbiota of patients with esophageal squamous cell carcinoma(EC)and healthy controls by using 16S rRNA gene sequencing technology,providing a reference for the treatment.Methods Fresh fecal specimens were obtained from 42 patients with EC,as well as 33 gender-and age-matched healthy controls,from Ju-ly 2023 to December 2023.The intestinal microbiota analysis was performed using 16S rRNA gene sequencing techno-logy.Results The results revealed significantly higher abundance of Proteobacteria and Actinobacteriota in EC group compared to NC control group.At the genus level,there was a significant increase in the abundances of Bifidobacterium,Escherichia-Shigella,Enterococcus,Blautia,Klebsiella,Collinsella and Bifidobacterium among patients with EC,while those of Bacteroides,Haemophilus,Dialister,Veillonella,Prevotella,Megamonas and Faecalibacterium were decreased.Conclusion 16S rRNA gene sequencing technology is expected to identify potential early warning factors related to the occurrence and progression of esophageal squamous cell carcinoma by monitoring changes within intestinal microbiota.
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