首页|In vivo anti-tumor activity of marine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene

In vivo anti-tumor activity of marine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene

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We have confirmed efficient anti-tumor activities of the peripheral lymphocytes transduced with a p185HER2-specific chimeric T-cell receptor gene both in murine and in human in our previous studies. To further test the feasibility of chimeric T-cell receptor in a bone marrow transplantation model, we first, made two murine tumor cell lines: MT901 and MCA-205, to express human p185HER2by retroviral gene transduction. Murine bone marrow cells were retrovirally transduced to express the chimeric T-cell receptor and gene-modified bone marrow cells were transplanted into lethally irradiated mouse. Six months post transplantation, p185HER2-positive tumor cells: MT-901/HER2 or MCA-205/HER2 was subcutaneously or intravenously injected to make mouse models simulating primary breast cancer or pulmonary metastasis. The in vivo anti-tumor effects were monitored by the size of the subcutaneous tumor or counting the tumor nodules in the lungs after India ink staining. The size of the subcutaneous tumor was significantly inhibited and the number of pulmonary nodules were significantly decreased in mouse recipients transplanted with chimeric T-cell receptor modified bone marrow cells compared with the control group. Our results suggest the efficient in vivo anti-tumor activities of chimeric T-cell receptor gene modified bone marrow cells.

Gene therapy Retrovirus Chimeric T-cell receptor Murine Hematopoietic stem cell Immunotherapy

JIAN MIN YANG、MICHAEL S FRIEDMAN、MARIANNE T HUBEN、JENNIFER FULLER、QIAO LI、ALFRED E CHANG、JAMES J MULE、KEVIN T MCDONAGH

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Department of Hematology, Changhai Hospital, the Second Military Medical University, Shanghai, P.R. China

Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, Michigan, USA

Department of Surgery, University of Michigan Medical School, Ann Arbor, Michigan, USA

Division of Hematology, Oncology, and Blood and Marrow Transplantation, Department of Internal Medicine, University of Kentucky College of Medicine, Lexington, Kentucky, USA

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2006

中华微生物学和免疫学杂志(英文版)
中华医学会

中华微生物学和免疫学杂志(英文版)

ISSN：1672-4666
年,卷(期)：2006.4(2)
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