散发性预激综合征与PRKAG2基因多态性的关系
Relationship between sporadic pre-excitation syndrome and PRKAG2 gene polymorphism
牛永红 1李小梅 1王晶 1张涛 1包敏 1张宴 1赵红 1黄立纲2
作者信息
- 1. 清华大学第一附属医院心脏中心,北京100016
- 2. 清华大学第一附属医院检验科,北京100016
- 折叠
摘要
目的 探讨散发性预激综合征与PRKAG2基因多态性的关系.方法 散发性预激综合征患者和健康受试者各65例,提取外周血白细胞基因组DNA,聚合酶链反应扩增PRKAG2基因片段,限制性片段长度多态性技术及DNA片段测序法检测基因多态性.结果 测序结果与NCBI Genebank BLAST软件进行对比,未发现突变点.结论 散发性预激综合征与PRKAG2基因多态性无关.
Abstract
Objective To explore the relationship between sporadic pre-excitation syndrome and PRKAG2 gene polymorphism. Methods Genomic DNA was isolated from peripheral lymphocytes obtained from 65 patients with sporadic pre-excitation syndrome and 65 normal persons. Mutation analysis was performed using purified Polymerase chain reaction(PCR) products to direct sequence analysis on PRKAG2. Results No mutation was identified in the 65 patients and 65 normal persons. Conclusion There is no relationship between PRKAG2 gene polymorphism and sporadic pre-excitation syndrome.
关键词
预激综合征/遗传学/多酶复合物/蛋白质丝氨酸苏氨酸激酶/多态现象,遗传Key words
Pre-Excitation Syndromes/genetics/Multienzyme Complexes/Protein-Serine-Threonine Kinases/Polymorphism, Genetic引用本文复制引用
基金项目
清华-裕元医学科学研究基金(20240000582)
出版年
2012
NETL
NSTL
维普
万方数据