Shh在散发性甲状腺髓样癌中的表达及其临床意义研究
Expression of Shh in sporadic medullary thyroid carcinoma and its clinical biological significance
边学海 1张广 1张大奇 1周乐 1李世杰 1孙辉1
作者信息
- 1. 吉林大学中日联谊医院甲状腺外科,吉林长春130033
- 折叠
摘要
目的 通过检测Sonic Hedgehog信号通路关键分子Shh在散发性甲状腺髓样癌表达情况,探讨其与散发性甲状腺髓样癌临床病理特征的关系及临床意义.方法 应用免疫组织化学方法检测11例甲状腺髓样癌患者癌组织及其癌旁组织病理蜡块标本中Shh的表达情况,分析其与临床病理特征的关系.结果 Shh蛋白主要表达于细胞膜与细胞质中.甲状腺髓样癌组织中Shh阳性表达率72.7％ (8/11)明显高于癌旁组织的9.1％(1/11).癌组织中Shh的表达与患者性别及年龄均无关(P＞0.05),而与肿瘤的TNM分期有关(P＜0.05).Shh在肿瘤直径≥1 cm和直径＜1 cm的表达阳性率分别为100％和20％,差异有统计学意义(P＜0.01).Shh在有淋巴结转移的肿瘤组织中和无淋巴结转移的肿瘤组织中的表达阳性率分别为100％和40％,差异有统计学意义(P＜0.05).结论 Shh信号通路的主要配体Shh蛋白在散发性甲状腺髓样癌中表达升高,并与肿瘤发生、分期及淋巴结转移有关,提示Shh信号通路的异常激活在散发性甲状腺髓样癌中发挥一定的作用,Shh蛋白为散发性甲状腺髓样癌预后判断指标及潜在的治疗靶点.
Abstract
Objective To define the expression of Shh,the key elements of Hedgehog signaling pathway in sporadic medullary thyroid carcinoma (SMTC) and to explore the relationship between the expression of Shh and clinical significance. Methods The expression of Shh was examined in 11 cases of SMTC tissues and corresponding adjacent-tumor thyroid tissues as control by immunohistochemistry. Analyze the relationship between the expression of Shh and clinical characteristics of SMTC patients. Results The positive rate of the cytoplasm of Shh expression was 72. 7%. Significant difference was found between normal thyroid tissues and SMTC. The research showed that the expression of Shh was related to the tumor size,clinical stages and lymph node metastasis, Shh was more significant in the tumor size( P<0. 01). Conclusions Varying expression of the main ligand Shh in Hedgehog signaling pathway was found in SMTC. The expression of Shh was related to the tumor size,clinical stage and lymph node metastasis, indicating that the aberrant activation of Shh signaling pathway plays some role in SMTC. Shh may be indicators for prognosis and ideal targets for therapy against SMTC.
关键词
癌,髓样/代谢/甲状腺肿瘤/代谢/猬蛋白质类/代谢Key words
Carcinoma, Medullary/metabolism/Thyroid Neoplasms/metabolism/Hedgehog Proteins/metabolism引用本文复制引用
基金项目
吉林省科技厅青年科研基金(201101046)
出版年
2012
NETL
NSTL
维普
万方数据