Effect of Anti-proliferation of tea polyphenols on human cervical carcinoma HeLa cells and its mechanism
Objective To investigate the effect of Tea Polyphenols(TP) on the proliferation of human cervical carcinoma HeLa cells and its possible mechanism. Methods The effect of TP on the growth of cervical carcinoma HeLa cells was tested by MTI assay. Fluorescent staining with Hochest 33258 and agarose gel electrophoresis were used to analyze the apoptosis. The expressions of Bcl2, Bax and P53 were tested by western blotting. Results The inhibition rate of HeLa cells elevated after 48h treatment with different concentrations of TP. Nuclei condensation and cleavage can be detected by Fluorescent staining. The formation of typical "DNA ladder" can be observed by agarose gel electrophoresis. Western blot showed that TP decreased the expressions of Bcl2 protein and increased the expressions of Bax and P53 protein in a does dependent manner. Conclusions TP can significantly inhibit the proliferation of human cervical carcinoma cells and induce cell apoptosis. Its possible mechanism is likely related to increasing the P53 and Bax levels and decreasing the Bcl2 level.
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万方数据
维普
