Application of hepatitis B virus genotyping and drug-resistance mutation genes detecting in Chaozhou area
Objective To evaluate the clinical significance of hepatitis B virus genotype and drug resistance genes mutation detection in Chaozhou. Methods The PCR-RDB was used to detect hepatitis B virus genotypes and drug resistance genes mutations in CHB patients. Adefovir was selected to therapy the patients with Lamivudine resistance gene mutations, the HBV DNA load and ALT content were detected again one year later. Results The infection rate of HBV B,C and B+D were 92. 31% (120/130) ,5. 38%(7/130) ,3. 21% (3/130) respectively. Lamivudine resistance mutation genes were detected in 24 persons,who were all HBV B genotype infectors. The rate of drug-resistance mutation was 18. 46% (24/130). Significant differences were found between the HBV B and the HBV C infectors when their HBV DNA load and ALT content were compared. Moreover, the HBV DNA load and ALT content of the Adefovir receptor were significantly decreased than one year ago. Conclusions HBV B is the most common HBV genotype in Chaozhou. Drug-resistance genes mutation is one of key factor that influence the CHB development.
NETL
NSTL
万方数据
维普
