HIF-1α和VEGF在骨肉瘤组织中的表达及其与血管生成的关系
The Relation of HIF-1α, VEGF Expression and Angiogenesis in Osteosarcoma
廉红星 1赵敏 1刘瑞吉1
作者信息
- 1. 秦皇岛市第一医院,河北秦皇岛066000
- 折叠
摘要
目的 观察HIF-1α和VEGF在骨肉瘤组织中的表达,并分析两者相关性,同时探讨其在骨肉瘤血管形成中所起的作用.方法 应用免疫组织化学SP法,检测35例骨肉瘤、14例骨软骨瘤中HIF-1α和VEGF的表达情况,同时用CD34标记血管内皮细胞,计算微血管密度(MVD).结果 35例骨肉瘤组织中,HIF-1α蛋白的阳性表达率为34.3％(12/35),与对照组相比差别有非常统计学意义(P＜0.01)；VEGF的阳性表达率为82.9％(29/35),亦显著高于对照组(P＜0.01); HIF-1α和VEGF的表达同时呈阳性,13例(37.1％)同时呈阴性,两者表达显著相关(r=0.589,P=0.000),且与癌组织分期显著相关(P＜0.05)；HIF-1α和VEGF均阳性的骨肉瘤组织中MVD值高于两者均为阴性者,两者相比较差异有非常统计学意义(P＜0.01).结论 HIF-1α和VEGF在骨肉瘤组织中均为高表达,提示它们可能对骨肉瘤新生血管生成中具有协同作用,在骨肉瘤的发生、发展起到重要作用.
Abstract
Objective To investigate the role of HIF-lα and VEGF in angiogenesis of osteosarcoma by analyze their expression. Methods The expression of HIF-la and VEGF specimens of 35 osteosarcoma and 14 osteochondroma tissues was evaluated by immunohistochemistry. MVD was calculated by using CD34 as the specific marker of mi-crovessel endothelium. Results The positive rat(34. 3% , 82. 9%) of HIF-la and VEGF in osteosarcoma were significantly higher than those in osteochondroma tissues (P<0. 01). The expressions of HIF-la and VEGF in osteosarcoma were positively correlated) P<0. 05). The expression of HIF-lα and VEGF were correlated with Staging (P<0. 05). The MVD was higher in the positive group of H1F-1α and VEGF than their negative group(P<0. 01). Conclusions The positive expression of HIF-la and VEGF are related with osteosarcoma microvascular generate. It seems that HIF-la and VEGF contribute to development of osteosarcoma.
关键词
骨肉瘤/代谢/缺氧诱导因子1,α亚基/代谢/血管内皮生长因子A/代谢/新生血管化,病理性Key words
Osteosarcoma/metabolism/Hypoxia-Inducible Factor 1, alpha Subunit/metabolism/Vascular Endothelial Growth Factor A/metabolism/Neovascularization, Pathologic引用本文复制引用
出版年
2012
NETL
NSTL
维普
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