Genotyping of Hepatitis C Virus among Drug Addict Population in Guangzhou
Objective To investigate the distribution of Hepatitis C virus (HCV) genotypes in population of drug abusers in Guangzhou. Methods 586 case sera samples of drug abusers were collected. Antt-HCV antibodies of these samples were detected by enzyme-linked immunoadsordent assay. The genotypes of HCV were conducted by reverse transcriptase nest polymerase chain reaction(RT-nPCR) and deoxyribonucleic acid sequeneeing of RT-nPCR products. Results Among the 586 drug abusers, the positive rate of anti-HCV antibody was 40.1 % (235/586). 218 cases of RT-nPCR positive were sequehced and analyzed. We detected five genotypes and the most predominant genotype was subtype lb( accounted for 63. 3%, 138/218), followed by 6a( 16. 5%),2a(11. 5% ), 3b(6.4%) and 3a (2. 3%). Conclusions The predominant genotype of HCV in drug abusers of Guangzhou was subtype lb, followed by 6a.
NETL
NSTL
万方数据
维普
