Effects of umbilical cord blood mesenchymal stem cells transplantation on the expression of NGF and BDNF in a rat cerebral infarction model
Objective To observe effects of umbilical cord blood mesenchymal stem cells (UCBMSCs) transplantation on the expression of NGF and BDNF and neural function in a rat cerebral infarction model. Methods The rats were randomly divided into into sham-operation group (n = 6), control group {n = 6) and transplantation group (n = 6). The rat models of middle cerebral artery occlusion (MCAO) were established with thread oceluders. The rats in transplantation group were injected via tail vein with 2× 105 UCBMSCs individually at the 1st day, 4 th day. The rats in sham-operation group and control group were injected with isopyknic PBS individually at the 1st day, 4 th day. Neurological score tests were performed at the 7th day and 14th day after transplantation. The expressions of NGF,BDNF in brain tissue were detected by immunohistochemistry technique at the 3rd day,7th day and 14th day after transplantation. Results The positive cells of NGF,BDNF expressions in control group and transplantation group were higher significantly than that in sham-operation group, the positive cells in transplantation group were higher significantly than that in control group at the 3rd day after transplantation. The positive cells gradually descend. There was no significant difference in the positive cells of NGF,BDNF expressions and neurological score between control group and transplantation group at the 14th day. Conclusions The transplantation of UCBMSCs early promoted the expression of NGF and BDNF, but its effects on nerve restoration was limited.
NETL
NSTL
万方数据
维普
