P57、P63和Ki-67在水肿性绒毛中的鉴别诊断意义
Expression and significance of P57, P63 and Ki-67 in the placental villi with hydropic changes
肖桂香 1王成鑫 2镇鸿燕 2陈莹 2刘丽江3
作者信息
- 1. 江汉大学医学院第二附属医院病理科,湖北武汉430050
- 2. 江大病理诊断所,湖北武汉430056
- 3. 江汉大学医学院第二附属医院病理科,湖北武汉430050;江大病理诊断所,湖北武汉430056
- 折叠
摘要
目的 探讨P57、P63和Ki-67在水肿性绒毛中的表达及其意义.方法 利用免疫组织化学技术检测23例部分性水泡状胎块(partial hydatidiform moles,PHM)、20例完全性水泡状胎块(complete hydatidiform moles,CHM)及13例早期水肿性流产的活检组织进行检查.结果 P57在完全性水泡状胎块中不表达(19/20)或仅微弱表达(1/20);相反,在所有的部分性水泡状胎块中,细胞滋养层细胞及绒毛间质细胞均表达P57(23/23),且13例呈中等至强阳性表达；在大部分水肿性流产中,P57均表达(11/13).P57在CHM与PHM、CHM与HA之间的表达差异均有统计学意义(P＜0.01).Ki-67在水泡状胎块中的表达明显高于水肿性流产(P＜0.05).无论是阳性细胞数量还是阳性程度,P63在三种病变中的表达均无明显差异.结论 P57和Ki-67在水肿性绒毛中的表达与其临床分型显著相关,运用免疫组织化学染色的方法,联合检测P57和Ki-67对鉴别不同类型的水肿性绒毛具有临床意义.P63在水肿性绒毛中的作用尚待进一步研究.
Abstract
Objective To investigate the expression and significance of P57, P63 and Ki-67 in the placental villi with hydropic changes. Methods 23 partial hydatidiform moles (PHM) patients, 20 complete hydatidiform moles (CHM) patients and 13 first trimester hydropic abortions patients were evaluated by means of immunohistochemical technique in this study. Results The results of immunohistochemical staining showed that P57 expression in cyto-trophoblasts was either absent (19 cases) or very low (1 case) in CHM. In contrast, in all PHM, nuclear PS7 was expressed in cytotrophoblasts and villous stromal cells (23/23). Meanwhile, P57 was moderately and strongly expressed in 13 cases. In the majority of hydropic abortions (11/13), P5 7 expression was observed. There was a significant difference between CHM and PHM, CHM and HA(P< 0. 01). The Ki-67 immunoreactivity was significantly higher in the hydatidiform moles group than in the abortion group with hydropic changes (P<0. 05). No matter the quantity of immunostaining or the intensity of immunostaining, there was no significant difference in expression of P63 among hydropic abortion, PHM and CHM. Conclusion Our findings suggest that expression of PS7 and Ki-67 can be used to differentiate between abortion with hydropic changes and hydatidiform moles when two markers are used together by means of immunohistochemical technique. The role of P63 in the placental vtUi with hydropic changes, however, further investigation is required.
关键词
绒毛膜绒毛/代谢/膜蛋白质类/代谢/周期素依赖激酶抑制剂p57/代谢/Ki-67抗原/代谢/诊断,鉴别Key words
Chorionic Villi/metabolism/Membrane Proteins/metabolism/Cyclin-Dependent Kinase Inhibitor p57/me-tabolism/Ki-67 Antigen/metabolism/Diagnosis, Differential引用本文复制引用
出版年
2012
NETL
NSTL
维普
万方数据