MiRNA-146a对血管平滑肌细胞凋亡的抑制作用
Essential role of miRNA-146a in apoptosis of vascular smooth muscle cell
骆瑜 1谢曼英 2熊玮2
作者信息
- 1. 暨南大学第二临床医学院深圳市人民医院老年病科,广东深圳518020
- 2. 暨南大学第二临床医学院深圳市人民医院急诊科,广东深圳518020
- 折叠
摘要
目的 观察miRNA-146a对血管平滑肌细胞凋亡的作用并研究其机制.方法 原代培养大鼠血管平滑肌细胞,分成inhibitor组、control组和normal组,采用脂质体2000分别转染miRNA-146a inhibitors(50 μM)、错义链(50 μM)、PBS,real time PCR测定转染后miRNA-146a水平,流式细胞仪检测转染后血管平滑肌细胞凋亡,western blot检测转染后Bax蛋白水平.结果 转染48 h后,inhibitor组血管平滑肌细胞的miRNA-146a水平明显低于normal和control组(P＜0.01),inhibitor组血管平滑肌细胞的凋亡显著高于normal、control组(P＜0.05),且Bax蛋白表达水平增加(P＜0.05).结论 miRNA-146a可以抑制血管平滑肌细胞的凋亡,其机制与抑制Bax表达相关.
Abstract
Objective To observe the role of miRNA-146a in apoptosis of vascular smooth muscle cell and investigate its mechanisms. Methods Primary rat vascular smooth muscle cells were cultured .artificial synthesized miRNA-146 inhibitors(50 μM),scrambles(50 fiM) and PBS were transfected into vascular smooth muscle cell by Lipofectamine 2000 individually. After transfection,flow cytometry was employed to measured apoptosis of vascular smooth muscle cell; Western Blot was employed to measure protein level of Bax in VSMC. Results After transfection 48h,compared with normal and control VSMCs, significantly lower miRNA-146a was observed in inhibitor VSMCs(P<0. 01). Meanwihle,inhibitor VSMCs showed higher apoptotic ability(P<0. 05) as well as higher protein level of Bax{P< 0.05}. Conclusions MiRNA-146a is capable of suppressing apoptosis of VMSC.which is probably related with that Bax protein was inhibited.
关键词
微RNAs/肌,平滑,血管/细胞学/细胞凋亡Key words
MicroRNAs/Muscle, Smooth, Vascular/cytology/Apoptosis引用本文复制引用
出版年
2012
NETL
NSTL
维普
万方数据