Essential role of miRNA-146a in apoptosis of vascular smooth muscle cell
Objective To observe the role of miRNA-146a in apoptosis of vascular smooth muscle cell and investigate its mechanisms. Methods Primary rat vascular smooth muscle cells were cultured .artificial synthesized miRNA-146 inhibitors(50 μM),scrambles(50 fiM) and PBS were transfected into vascular smooth muscle cell by Lipofectamine 2000 individually. After transfection,flow cytometry was employed to measured apoptosis of vascular smooth muscle cell; Western Blot was employed to measure protein level of Bax in VSMC. Results After transfection 48h,compared with normal and control VSMCs, significantly lower miRNA-146a was observed in inhibitor VSMCs(P<0. 01). Meanwihle,inhibitor VSMCs showed higher apoptotic ability(P<0. 05) as well as higher protein level of Bax{P< 0.05}. Conclusions MiRNA-146a is capable of suppressing apoptosis of VMSC.which is probably related with that Bax protein was inhibited.
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万方数据
维普
