首页|Stat3、HIF-1α、MMP-7和VEGF在肾母细胞瘤中的表达

Stat3、HIF-1α、MMP-7和VEGF在肾母细胞瘤中的表达

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目的 研究血管内皮生长因子(vascular endothelial growth factor,VEGF),信号传导与转录激活因子3(signal transduccer and activator of transcription 3,Stat 3)、低氧诱导因子HIF-1α(hypoxia-inducible factor-1α,HIF-1α)和基质金属蛋白酶-7(MMP-7)在肾母细胞瘤(nephrohlastoma,Wilms'tumor,WT)中的表达及其及其与临床分期和病理分级间的关系.方法 采用免疫组化SP法检测43例手术切除WT标本和24例小儿非肿瘤肾组织标本中Stat 3、HIF-1α、MMP-7、与VEGF的表达.结果 24例对照组织标本中MMP-7未见表达,43例WT组织标本中MMP-7在胞浆表达率54.18±10.72(MVD值).MMP-7在预后良好(favorable histological types,FH)组中的阳性表达率明显低于在预后不良(unfavorable histological types,UH)组中的阳性表达率(P＜0.05); VEGF、Star 3及HIF-1α在WT组织中表达强度较正常肾组织显著增强(P＜0.05).另外,临床分期Ⅲ～Ⅳ和预后不良病理类型的WT中Star 3及VEGF表达强度明显高于临床分期Ⅰ～Ⅱ的WT,预后不良病理类型及直径≥6 cm的WT中HIF-1α表达强度较预后良好型及直径＜6cm的WT升高.VEGF的表达与性别、年龄和病理学分型无关(P＞0.05),而与临床分期明显相关(P＜0.05).结论 WT组级中Stat 3、HIF-1α、MMP-7和VEGF的表达与肿瘤侵袭转移和发展有关,可被认为是肾母细胞侵袭性标志,对WT对于靶向治疗肾母细胞瘤具有一定意义.
Stat3, HIF-1α, MMP-7 and VEGF in Wilms tumor expression and clinical significance
Objective Detection of Wilms tumor (nephrohlastoma, wilms tumor, WT) tissues of Stat3, HIF-lα, MMP-7and VEGF expression and clinical stage and pathological grade relationships. Methods lmmunohistochemical SP method detected 43 cases of surgical specimens and 24 eases of children with WT non-tumor kidney tissues Stat3, HIF-la,MMP-7and VEGF expression. Results 24 cases in control tissues did not express MMP-7, 43 cases of WT tissue samples MMP-7 expression in the cytoplasm of 54.18±10. 72(MVD). MMP-7 in a good prognosis (favorable histological types, FH) positive expression group was significantly lower than in the poor prognosis (unfavorable histological types, UH) group, positive expression rate (P<0. 001); The expression of Stat3, HIF-lo and VEGF were significantly up-regulated in Wilms'tumor tissues compared to those in adjacent tissues and normal kidney tissues (P<0. 05). Stat3 and VEGF proteins in Wilms'tumor tissues of Stage Ⅲ～Ⅳ were significantly higher than those of stage Ⅰ～Ⅱ The higher expression of HIF-1 in Wilms'tumor tissues was shown in tumors with high risk histopathology . VEGF in Wilms tumor tissues was significantly higher than children of non-tumor kidney tissue (P <0. 05). VEGF expression significantly correlated with the clinical stage (P<0.05). Conclusions WT tissue Stat3, H1F-1.MMP7 and VEGF expression and tumor invasion, metastasis and development, can be considered invasive signs of Wilms on WTs clinical stage and pathologic grade and prognosis significance.

Wilms TumormetabolismSTAT3 Transcription FactormetabolismHypoxia-lnducible Factor 1, alpha SubunitmetabolismMatrix Metalloproteinase 7metabolismVascular Endothelial Growth Factor Ametabolism

宗国兰、杜春林、刘秉成、刘小伶、钟小梅、魏艳红、张歆萍、关艳

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承德医学院附属医院小儿外科,河北承德067000

承德市中心医院

Wilms瘤/代谢 STAT3转录因子/代谢 缺氧诱导因子1,α亚基/代谢 基质金属蛋白酶7/代谢 血管内皮生长因子A/代谢

河北省承德市科学技术研究与发展计划项目

200922112

2012

中国误诊学杂志
中华预防医学会　漯河市中心医院　重庆第九人民医院

中国误诊学杂志

影响因子：0.406
ISSN：1009-6647
年,卷(期)：2012.12(18)
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