Study on the disinfection effect of a new compound disinfectant
Objective To study the efficacy and mechanism of a new compound disinfection in killing different types of microorganisms.Methods The suspension quantitative bactericidal test method was used to study the bactericidal effect of the disinfectant;real-time fluorescence quantitative PCR and transmission electron microscopy were used to study the killing mechanism of the disinfectant on microorganisms.Results The disinfectant acted on Escherichia coli and Pseudomonas aeruginosa for 30 s and could kill them all;on Staphylococcus aureus for 30 s,the killing log value>5.00;on spores of Bacillus subtilis var niger for 60 min,the killing log value was 1.19.After Escherichia coli were trented by the compound disinfectant the expression levels of membrane protein genes LptD,LptE,OmpA,OmpC were slightly increased compared to the control group;the cell membrane of Escherichia coli and Staphylococcus aureus were damaged by the compound disinfectant.Conclusion This new compound disinfectant can effectively kill bacterial propagules mainly by destroying bacterial cell membrane and oxidizing intracellular substances to kill microorganisms.
compound disinfectantmechanism of actiondisinfection effect
NETL
NSTL
万方数据
维普
