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牛血清白蛋白对臭氧水杀菌效果的影响观察

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目的 观察不同浓度牛血清白蛋白(BSA)对臭氧水杀菌效果的影响.方法 采用悬液定量杀菌试验方法,分别检测臭氧水在不同浓度BSA条件下对金黄色葡萄球菌和大肠埃希菌的杀菌效果.结果 在无BSA干扰下,臭氧浓度为(10.5±0.5)mg/L的臭氧水对金黄色葡萄球菌作用30 min的平均杀灭对数值为4.85;在0.3%、3%BSA条件下,臭氧浓度为5.00~11.00 mg/L的臭氧水对金黄色葡萄球菌作用30 min的最大平均杀灭对数值为0.87.在无BSA干扰下,臭氧浓度为(5.5±0.5)mg/L的臭氧水对大肠埃希菌作用0.5 min的平均杀灭对数值为5.83;在0.3%、3%BSA条件下,臭氧浓度为5.00~11.00 mg/L臭氧水对大肠埃希菌作用1.5 min的最大平均杀灭对数值为1.20.结论 臭氧消毒机产生的臭氧水在无BSA干扰下,5.00 mg/L对大肠埃希菌杀菌效果即能达到合格要求;在无或有BSA条件下,5.00~11.00 mg/L臭氧水对金黄色葡萄球菌杀菌效果均未能达到合格要求.
Observation on the influence of bovine serum albumin on the bactericidal effect of ozone water
Objective To observe the effect of different concentrations of bovine serum albumin(BSA)on the bactericidal effect of ozone water.Methods The suspension quantitative bactericidal test method was used to detect the bactericidal effect of ozone water on Staphylococcus aureus and Escherichia coli under different concentrations of BSA.Results Without the interference of BSA,the average killing log value of ozone water with ozone concentration of(10.5±0.5)mg/L against Staphylococcus aureus for 30 min was 4.85;under the conditions of 0.3%and 3%BSA,the maximum average killing log value of ozone water with ozone concentration of 5.00-11.00 mg/L against Staphylococcus aureus for 30 min was 0.87.Without the interference of BSA,the average killing log value of ozone water with ozone concentration of(5.50±0.5)mg/L against Escherichia coli for 0.5 min was 5.83;under the conditions of 0.3%and 3%BSA,the maximum average killing log value of ozone water with ozone concentration of 5.00-11.00 mg/L against Escherichia coli for 1.5 min was 1.20.Conclusion The ozone water produced by the ozone disinfection machine can meet the qualified requirements for killing Escherichia coli at a concentration of 5.00 mg/L without BSA interference;under the conditions of without or with BSA,the bactericidal effect of 5.00—11.00 mg/L ozone water on Staphylococcus aureus can't meet the qualified requirements.

ozone waterbactericidal effectbovine serum albuminbacterial propagule

张雪、蔡增宅、任哲、韩杰、魏秋华

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中国人民解放军疾病预防控制中心,北京 100071

臭氧水 杀菌效果 牛血清白蛋白 细菌繁殖体

生物安全研究专项

20SWAQX40

2024

中国消毒学杂志
军事医学科学院疾病预防控制所,中华预防医学会

中国消毒学杂志

CSTPCD
影响因子:0.754
ISSN:1001-7658
年,卷(期):2024.41(1)
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