Comparation of different methods on disinfectant efficacy evaluation of quaternary ammonium salt compound wipes
Objective To evaluate the disinfectant efficacy of compound quaternary ammonium salt wipes.Methods Disinfectant wipes were prepared by impregnation with a solution containing 2 060 mg/L of benzalkonium chloride,which concentration of the squeezed solution of wipes was 1 800 mg/L.The disinfection effect of the wipe was evaluated by suspension quantitative germicidal test,carrier quantitative germicidal test,wipes carrier quantitative germicidal test and field disinfection test.Results For E.coli,S.aureus and P.aeruginosa in suspension incubated with the squeezed solution of wipes for 1 min,the killing logarithmic(KL)values were＞5.00 and that of C.albicans was＞4.00.For E.coli,S.aureus and P.aeruginosa on the cloth carrier,when incubated with the disinfectant for 5 min,the KL values were 3.18,3.28 and 3.12,respectively,and that of C.albicans was 1.22 with 10 min incubation.For E.coli,S.aureus and P.aeruginosa in tryptone physiology solution plus 3%organic substance,the KL values were 3.58,3.86 and 3.42,respectively,and that of C.albicans was 2.89 when incubated for 10 min.For E.coli,S.aureus and P.aeruginosa in phosphate buffered saline,the mean KL values were 3.92,5.30 and 3.80,respectively when incubated for 1 min,and that of C.albicans was 3.22 when incutated for 3 min.The KL values of wipes against natural bacteria on surface of desk were all＞1.00.Conclusion There are significant differences in the results of different experimental methods on disinfectant efficacy evaluation.
万方数据
维普
