摘要
目的 观察不同试验方法对季铵盐类消毒湿巾消毒效果的评价.方法 用含苯扎氯铵2 060 mg/L的溶液浸渍制备消毒湿巾,其挤出液苯扎氯铵为1 800 mg/L.采用悬液定量杀菌试验、载体定量杀菌试验、湿巾载体定量杀菌试验、现场消毒试验等方法对季铵盐类消毒湿巾的消毒效果进行综合评价.结果 湿巾挤出液对悬液内指示细菌(大肠埃希菌、铜绿假单胞菌、金黄色葡萄球菌)作用1 min,杀灭对数值(KL)均＞5.00,对白色念珠菌作用1 min,KL＞4.00;湿巾挤出液对布片载体上的指示细菌作用5 min,KL均值分别为3.18、3.28和3.12,对布片载体上的白色念珠菌作用10 min,KL均值为1.22.湿巾载体对TPS与等量3%有机干扰物配制的指示细菌溶液作用3 min,KL值分别为3.53、3.86和3.42,对白色念珠菌作用10 min,KL值为2.89;湿巾载体对PBS配制的指示细菌悬液作用1 min,KL均值分别为3.92、5.30和3.80,对白色念珠菌作用3 min,KL均值为3.22.湿巾擦拭对桌面自然菌的KL值＞1.00.结论 不同的试验方法结果差异较大,根据消毒湿巾的实际使用情况,建议采用湿巾载体定量杀菌试验、现场消毒试验来综合评价消毒效果.
Abstract
Objective To evaluate the disinfectant efficacy of compound quaternary ammonium salt wipes.Methods Disinfectant wipes were prepared by impregnation with a solution containing 2 060 mg/L of benzalkonium chloride,which concentration of the squeezed solution of wipes was 1 800 mg/L.The disinfection effect of the wipe was evaluated by suspension quantitative germicidal test,carrier quantitative germicidal test,wipes carrier quantitative germicidal test and field disinfection test.Results For E.coli,S.aureus and P.aeruginosa in suspension incubated with the squeezed solution of wipes for 1 min,the killing logarithmic(KL)values were＞5.00 and that of C.albicans was＞4.00.For E.coli,S.aureus and P.aeruginosa on the cloth carrier,when incubated with the disinfectant for 5 min,the KL values were 3.18,3.28 and 3.12,respectively,and that of C.albicans was 1.22 with 10 min incubation.For E.coli,S.aureus and P.aeruginosa in tryptone physiology solution plus 3%organic substance,the KL values were 3.58,3.86 and 3.42,respectively,and that of C.albicans was 2.89 when incubated for 10 min.For E.coli,S.aureus and P.aeruginosa in phosphate buffered saline,the mean KL values were 3.92,5.30 and 3.80,respectively when incubated for 1 min,and that of C.albicans was 3.22 when incutated for 3 min.The KL values of wipes against natural bacteria on surface of desk were all＞1.00.Conclusion There are significant differences in the results of different experimental methods on disinfectant efficacy evaluation.
维普
万方数据