Verification of killing effect of electron beam on SARS-CoV-2
Objective To verify the inactivation capability of electron beam against SARS-CoV-2,and to provide a basis for application.Methods Vero cells were cultured to produce SARS-CoV-2 viral suspension.The viral suspension was dorped on filter paper then irradiated with various doses of electron beam.The titer of the surviving virus and the nucleic acid of SARS-CoV-2 in the recovered supernatant was detected.The killing effect was estimated by nucleic acid test,cytopathic effect and the half tissue infection amount of the virus(TCID50).Results At-20℃to 20℃,the killing logarithm value of 5 kGy irradiation dose for SARS-CoV-2 was>4.0.At-80℃,the killing logarithm value of 9 kGy irradiation dose for SARS-CoV-2 were>4.0.Conclusion For the lower temperature,the tolerance dose of SARS-CoV-2 to the electron beam is higher.