首页|人冠状病毒HCoV-229E在无生命物体表面存活时间比较研究

人冠状病毒HCoV-229E在无生命物体表面存活时间比较研究

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目的 比较人冠状病毒HCoV-229E在不同的无生命载体上的存活时间,为做好冠状病毒消毒提供依据.方法 试验环境温度为(25±1)℃,相对湿度为55%±10%,采用细胞接种法检测病毒存活,病毒悬液滴度达到国家标准要求,进行不同物体表面上HCoV-229E的存活试验.结果 纸片、布片载体上病毒滴度下降较快,滴染纸片、布片72 h后,病毒滴度接近0;塑料片载体上病毒滴度下降较慢,放置5 d病毒滴度为0.75 logTCID50/0.1 mL;不锈钢载体、玻璃片载体上病毒滴度下降较慢,放置4 d后,病毒滴度分别为1.0和0.75 logTCID50/0.1 mL.5 d以后所有载体上均未检测到活病毒.结论 HCoV-229E在不同无生命物体表面上存活时间不同,在实际消毒工作中应有针对性地开展物体表面和环境消毒,提高消毒工作效能.
Comparative study on the survival time of human coronavirus HCoV-229E on the surface of inanimate objects
Objective To compare the survival time of human coronavirus HCoV-229E on different vectors of inanimate object surfaces,and to provide evidence for coronavirus disinfection.Methods Under the conditions of test ambient temperature of(25±1)℃and relative humidity of 55%±10%,the cell inoculation method was used to detect virus survival and the titer of virus suspension reached the national standard,and the survival test of HCoV-229E on different object surfaces was conducted.Results The virus titer on the vectors of paper and cloth decreased rapidly,and was close to 0 after 72 h of instilled contamination of paper and cloth.The virus titer decreased slowly on the plastic vector,and was 0.75 logTCID50/0.1 mL after 5 d.The virus titer on the vectors of stainless steel and glass sheet decreased slowly,and was 1.0 logTCID50/0.1 mL and 0.75 logTCID50/0.1 mL respectively after 4 days,and no live virus was detected on all vectors of inanimate object surfaces after 5 d.Conclusion The survival times of HCoV-229E on different inanimate object surfaces are different,and targeted disinfection of object surfaces and environment should be carried out in actual disinfection work to improve disinfection efficiency.

human coronavirusvectorobject surfacesurvival time

刘冷、邓惠、陈惠珍、郑小凌、王冰姝、王雅静、黄旭和、张磊、纪迅敏、钟昱文

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广东省疾病预防控制中心,广东广州 511430

广东省生物制品与药物研究所

人冠状病毒 载体 物体表面 存活时间

广东省医学科研基金

202342793455200

2024

中国消毒学杂志
军事医学科学院疾病预防控制所,中华预防医学会

中国消毒学杂志

CSTPCD
影响因子:0.754
ISSN:1001-7658
年,卷(期):2024.41(4)
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