人冠状病毒HCoV-229E在无生命物体表面存活时间比较研究
Comparative study on the survival time of human coronavirus HCoV-229E on the surface of inanimate objects
刘冷 1邓惠 1陈惠珍 1郑小凌 1王冰姝 1王雅静 1黄旭和 1张磊 2纪迅敏 2钟昱文1
作者信息
- 1. 广东省疾病预防控制中心,广东广州 511430
- 2. 广东省生物制品与药物研究所
- 折叠
摘要
目的 比较人冠状病毒HCoV-229E在不同的无生命载体上的存活时间,为做好冠状病毒消毒提供依据.方法 试验环境温度为(25±1)℃,相对湿度为55%±10%,采用细胞接种法检测病毒存活,病毒悬液滴度达到国家标准要求,进行不同物体表面上HCoV-229E的存活试验.结果 纸片、布片载体上病毒滴度下降较快,滴染纸片、布片72 h后,病毒滴度接近0;塑料片载体上病毒滴度下降较慢,放置5 d病毒滴度为0.75 logTCID50/0.1 mL;不锈钢载体、玻璃片载体上病毒滴度下降较慢,放置4 d后,病毒滴度分别为1.0和0.75 logTCID50/0.1 mL.5 d以后所有载体上均未检测到活病毒.结论 HCoV-229E在不同无生命物体表面上存活时间不同,在实际消毒工作中应有针对性地开展物体表面和环境消毒,提高消毒工作效能.
Abstract
Objective To compare the survival time of human coronavirus HCoV-229E on different vectors of inanimate object surfaces,and to provide evidence for coronavirus disinfection.Methods Under the conditions of test ambient temperature of(25±1)℃and relative humidity of 55%±10%,the cell inoculation method was used to detect virus survival and the titer of virus suspension reached the national standard,and the survival test of HCoV-229E on different object surfaces was conducted.Results The virus titer on the vectors of paper and cloth decreased rapidly,and was close to 0 after 72 h of instilled contamination of paper and cloth.The virus titer decreased slowly on the plastic vector,and was 0.75 logTCID50/0.1 mL after 5 d.The virus titer on the vectors of stainless steel and glass sheet decreased slowly,and was 1.0 logTCID50/0.1 mL and 0.75 logTCID50/0.1 mL respectively after 4 days,and no live virus was detected on all vectors of inanimate object surfaces after 5 d.Conclusion The survival times of HCoV-229E on different inanimate object surfaces are different,and targeted disinfection of object surfaces and environment should be carried out in actual disinfection work to improve disinfection efficiency.
关键词
人冠状病毒/载体/物体表面/存活时间Key words
human coronavirus/vector/object surface/survival time引用本文复制引用
基金项目
广东省医学科研基金(202342793455200)
出版年
2024
NETL
NSTL
万方数据