Investigation on the contamination status and disinfectant resistance of Staphylococcus aureus in public elevators
Objective To investigate the contamination status of Staphylococcus aureus on public elevator buttons of hospitals and hotels,and to observe the resistance of Staphylococcus aureus to commonly used disinfectants.Methods Baird-Parker screening medium was used to isolate Staphylococcus aureus,and the disinfectant resistance genes qacA/B,smr were amplified by polymerase chain reaction.The resistance of Staphylococcus aureus to commonly used disinfectants was tested using minimal inhibitory concentration(MIC)and minimum bactericidal concentrations(MBC).Results A total of 21 strains of Staphylococcus aureus were detected on 83 public elevator buttons,with a detection rate of 25.3%.The detection rates of Staphylococcus aureus on the elevator buttons of hospitals and hotels were 10.0%and 39.5%,respectively,with a statistically significant difference(P=0.002).The genes qacA/B,smr were not detected in the 21 strains of Staphylococcus aureus isolated,but some strains showed higher resistance to quaternary ammonium disinfectants than the standard strains.Conclusion The contamination of hotel elevator buttons by Staphylococcus aureus is more severe than that of hospital elevator buttons,and it has been found that some Staphylococcus aureus had developed resistance to quaternary ammonium salt disinfectants.
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