Methods comparing of virus titer testing used in viral cleaning efficiency evaluation of cleaning device in ventilation system
Objective To compare three different virus titer testing methods in evaluating the virus aerosols cleaning efficiency of cleaning devices used in ventilation system.Methods After aerosol contamination,plaque assay,quantitative polymerase chain reaction(qPCR)and integrated cell culture-qPCR(ICC-qPCR)were used to detcted virus titer.One pass cleaning efficiency of 5 different types of cleaning devices were compared.Results For high voltage electrostatic purification device,primary efficiency filter and medium efficiency filter,cleaning efficiency had no significant difference among the 3 virus titer testing methods.For ultraviolet cleaning devices and high efficiency particulate air filter(P＞0.01),the test sencibility of qPCR method was higher than the other two methods(P＜0.01).ICC-qPCR method had the shortest time consuming(about 5 h)and had the best repeatability.RSD was lower than the other two methods,and the lowest value was＜0.01％.Conclusion ICC-qPCR method has a shorter test cycle and is more stable.It is suitable for evaluating virus cleaning efficiency of the cleaning devices used in ventilation system.
万方数据
维普
