Distribution characteristics and drug resistance correlation analysis of integron in clinically isolated Morganella morganii
Objective To investigate the distribution of integrons and the molecular characteristics of variable region resistance gene cassettes of integron-positive strains of Morganella morganii isolated from our hospital and their relationship with drug resistance.Methods Ninety-seven strains of non-replicated M.morganii clinically isolated from November 2015 to August 2021 at our hospital were collected and detected by PCR for class 1,2 and 3 integrons.Homology analysis of integron-positive strains was performed by ERIC-PCR.Results Among the 97M.morganii strains,28 strains were positive for class 1 integrons,accounting for 28.9％.Five strains were positive for class 2 integrons,accounting for 5.2％,of which one strain carried both class 1 and class 2 integrons,and no class 3 integrons were detected.PCR products were successfully amplified in the variable region of 26 of the 32 integron-positive strains.The drug resistance gene cassettes carried by the variable region of the class 1 integron-positive strains were mainly dfrA17-aadA5,and those carried by the variable regions of the class 2 integron-positive strains were mainly dfrA1-sat2-aadA1.The resistance rates of cotrimoxazole,ampicillin/sulbactam,ciprofloxacin were 90.6％,81.3％,78.1％,respectively,which were higher than those of integron-negative strains(P＜0.05).The 32 integron-positive M.morganii strains were mainly of two genotypes,A and B.Conclusion Variable regions of class 1 integrons in M.morganii mainly carry aminoglycoside and trimethoprim drug resistance gene cassettes and are highly correlated with host bacterial drug resistance,with high homology among integron-positive strains and possible intra-hospital clone and transmission.
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维普
