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银二氧化钛纳米材料的抑菌效果观察

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目的 了解银二氧化钛纳米材料(Ag/TiO2)对口腔常见致病菌的抑菌效果.方法 通过化学方法将Ag均匀沉积于TiO2NP25表面,获得Ag/TiO2纳米材料,并对其进行形貌特征及化学结构检测分析.通过检测最小抑菌浓度(MIC)和抑菌率,评价Ag/TiO2对口腔常见致病菌的抑菌效果.结果 结果表明,化学方法可以将Ag均匀沉积于TiO2的表面,TiO2的直径约50 nm,Ag的直径约5 nm.抑菌试验结果显示,合成Ag/TiO2在未光照的条件下对金黄色葡萄球菌、大肠埃希菌和白念珠菌的最小抑菌浓度均为250 mg/L.当浓度为1 000 mg/L时抑菌率可达94.3%,对大肠埃希菌和白念珠菌的抑菌率分别为77.9%和84.6%,2 000 mg/L的TiO2未能检测到对3种试验菌的抑菌作用.结论 本研究通过简单化学方法成功制备Ag/TiO2,且可有效抑制口腔常见致病菌.
Observation on bacteriostasis effect of silver/TiO2 nanomaterials
Objective To understand the bacteriostasis effect of silver/TiO2 nanomaterials(Ag/TiO2)on common oral pathogens.Methods Ag was uniformly deposited on the surface of TiO2NP25 by chemical methods,and the Ag/TiO2 composite nanomaterials were analyzed for the morphological characteristics and chemical structure detection of the synthetic materials.The bacteriostasis effect of the synthetic materials on common pathogenic bacteria was evaluated by detecting the minimum bactericidal concentration(MIC)and bacteriostasis rate.Results The results showed that Ag was uniformly deposit on the surface of TiO2,and the diameter of TiO2 was about 50 nm and the Ag was 5 nm.The results of bacteriostasis experiments showed that all the minimum inhibitory concentration of synthetic Ag/TiO2 against S.aureus,E.coli,and C.albicans were 250 mg/L.At the concentration of 1 000 mg/L,the inhibition rate reached 94.3%,77.9%and 84.6%for E.coli and C.albicans,respectively,and 2 000 mg/L TiO2 failed to detect the inhibition against the 3 strains of experimental bacterias.Conclusion In this study,Ag/TiO2 is successfully prepared by simple chemical methods and it can effectively inhibit common oral pathogens.

nanomaterialDUWLbacteriostasis effectbacteriostasis rate

珊丹、张智玲、孙冰雨、张友

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天津市口腔医院天津市口腔功能重建重点实验室,天津 300041

纳米材料 口腔综合治疗台水路 抑菌效果 抑菌率

2021年天津市口腔功能重建重点实验室面上项目

2021KLMS10

2024

中国消毒学杂志
军事医学科学院疾病预防控制所,中华预防医学会

中国消毒学杂志

CSTPCD
影响因子:0.754
ISSN:1001-7658
年,卷(期):2024.41(5)