Observation on the bactericidal effect of a double-chain quaternary ammonium salt disinfectant on surface of objects at low temperature
Objective To observe the disinfection effect of a double-chain quaternary ammonium salt disinfectant in a low-temperature environment of-40℃,and to provide reference for the application of low-temperature disinfection.Methods Carrier quantitative bactericidal test and on-site disinfection test methods were used to observe the disinfection effect of the double-chain quaternary ammonium salt disinfectant on environmental surfaces under low temperature conditions.Results The content of stock solution of the double-chain quaternary ammonium salt disinfectant was 37 g/L,and the pH value was 5.03.This disinfectant has no precipitation or crystallization after being placed at-40℃ for>8 h.In a low-temperature cold storage at-40℃,the stock solution was sprayed for 30 min,and the killing log values of Escherichia coli and Staphylococcus aureus on the fabric samples were both>3.00;the stock solution was sprayed at 10 mL/m3 for 60 min,and the killing log values of Escherichia coli and Staphylococcus aureus on the glass surface were both>3.00.The stock solution of the disinfectant was non-irritating to the intact skin of New Zealand rabbits.The acute oral toxicity test on mice was non-toxic and had no micronucleogenic effect on mice.Conclusion This double-chain quaternary ammonium salt disinfectant can kill Escherichia coli and Staphylococcus aureus on environmental surfaces at low temperature of-40℃,and is an actual non-toxic substance.
万方数据
维普
