Objective To analyze the molecular epidemiological characteristics of carbapenem resistant Klebsiella pneumoniae(CRKP).Methods CRKP strains isolated from the Inner Mongolia Autonomous Region People's Hospital from March 2022 to March 2023 were selected to conduct drug sensitivity test,pulsed field gel electrophoresis(PFGE)and whole genome sequencing(WGS).Results A total of 33 strains of CRKP were isolated,of which 81.81%were from sputum samples.CRKP had high resistance to most commonly used antibiotics,and was most sensitive to tigecycline,with a resistance rate of 3.03%.PFGE results showed that 33 strains of CRKP were divided into 26 clusters,of which 8 strains were in the same cluster.After WGS for the 8 strains,the obtained sequences were analyzed by whole genome SNP,and they were homology due to the maximum difference of 22 bases.Conclusion CRKP has been present in a hospital for a long time and has silent transmission.It is necessary to standardize the use of antibiotics,strengthen hospital infection prevention and control measures,and regularly monitor to prevent the outbreak of hospital infections.
关键词
耐碳青霉烯类肺炎克雷伯菌/药敏试验/脉冲场凝胶电泳/全基因组测序
Key words
carbapenem resistant Klebsiella pneumoniae/drug sensitivity test/pulsed field gel electrophoresis/whole genome sequencing
维普
万方数据