不同抑菌剂对洋葱伯克霍尔德菌的抑菌效力研究
Bacteriostasis potency of various antimicrobial agents against Burkholderia cepacia
魏兰芬 1陈冠群 2潘协商 1曹珍珍 2谢中艺 1许激1
作者信息
- 1. 浙江省疾病预防控制中心,浙江杭州 310051
- 2. 杭州高琦香精化妆品有限公司
- 折叠
摘要
目的 观察13种单方抑菌化合物和6种复配型抑菌剂对洋葱伯克霍尔德菌的抑菌效力.方法 采用试管内悬液法,测定洋葱伯克霍尔德菌在不同单方抑菌化合物及不同复配抑菌剂中的存活情况.结果 13种常用单方抑菌剂中,2.0%1,2-己二醇和0.08%十二烷基二甲基苄基氯化铵作用0.5 h,0.15%尼泊金甲酯作用24 h,0.10%尼泊金乙酯作用1 h,洋葱伯克霍尔德菌数量减少对数值均≥5.0,其余单方组分作用28 d杀灭对数值均≤3.0,部分抑菌剂在28 d测试周期内,残留菌有增加的现象.0.15%尼泊金乙酯、0.08%和0.12%的十六烷基氯化吡啶在作用体系中残留菌数<10 cfu/mL的情况下,菌数仍增加.0.05%辛酰羟肟酸+0.15%对羟基苯乙酮、0.13%十二烷基二甲基苄基氯化铵+0.06%十六烷基氯化吡啶等抑菌效力不符合ISO 11930《化妆品的抗菌保护性评价》要求,其他4种复配型抑菌效力符合ISO 11930要求.结论 单方抑菌剂对洋葱伯克霍尔德菌的抑菌效力可通过特定复配得到提升.
Abstract
Objective To observe the bacteriostasis potency of 13 individual antimicrobial agents and 6 compound liquids against Burkholderia cepacia.Methods The tube suspension method was used to monitor the survival of Burkholderia cepacia in various individual antimicrobial agents and compound liquids.Results Among the 13 commonly used individidual antimicrobial agents,a 2.0%solution of 1,2-hexanediol and a 0.08%dodecyl dimethyl benzyl ammonium chloride incubating for 0.5 h,0.15%methylparaben inccubating for 24 h,and 0.10%ethylparaben incubating for 1 h,the killing log value for Burkholderia cepacia decreased by≥5.0,and the killing log values of other individual components were≤3.0 after incubationg for 28 d.The number of bacteria in the test system of 0.15%ethylparaben,0.08%and 0.12%cetylpyridine chloride could still increase when the number of bacteria in the system<10 cfu/mL.The bacteriostasis efficacy of 0.05%caprylhydroxamic acid+0.15%p-hydroxyacetophenone and 0.13%dodecyl dimethyl benzyl ammonium chloride+0.06%cetylpyridine chloride did not meet the ISO 11930"Evaluation of the antimicrobial protection of a cosmetic product"requirements,and the bacteriostasis efficacy of the other four compounds met the requirements.Conclusion The bacteriostasis potency of individual antimicrobial agents against Burkholderia cepacia can be enhanced by specific compounding.
关键词
洋葱伯克霍尔德菌/抑菌效力/细菌/真菌Key words
Burkholderia cepacia/antibacterial potency/bacteria/fungi引用本文复制引用
出版年
2024
NETL
NSTL
万方数据