Application of vaporized hydrogen peroxide in terminal disinfection in animal biosafety level 3 laboratory
Objective To explore the optimal disinfection dosage and scheme of vaporized hydrogen peroxide on indoor air and high efficiency particulate air filter(HEPA)during terminal disinfection in animal biosafety level 3(ABSL-3)laboratory.Method The hydrogen peroxide at concentration of 5,8,10 and 15 g/m3 were used to disinfect different volumes of space,and the disinfection effect of vaporized hydrogen peroxide was verified by chemical indicator card and biological indicator bacteria,and its corrosiveness to indoor color steel plate was observed.Exhaust HEPA filters were disninfected in situ with 10,12 and 15 g/m3 of hydrogen peroxide which were screened for optimal dosage.Results The 10 g/m3 vaporized hydrogen peroxide could effectively disinfect the≤200 m3 laboratory space,and there was no corrosion of color steel plates.With the use of a disinfection circulating fan,15 g/m3 vaporized hydrogen peroxide could disinfect the laboratory tuyere-type exhaust HEPA filter in situ.Conclusion The disinfection scheme of vaporized hydrogen peroxide disinfection circulating fan can achieve the simultaneous disinfection of indoor air and exhaust HEPA filter during the termial disinfection of ABSL-3 laboratory.