Analysis of active ingredients and observation of microbial disinfection performance of electrocatalytic hydrogen peroxide disinfectant
Objective To analyze the active species in the process of electrocatalytic production of hydrogen peroxide and to observe their disinfection performance on three indicator bacteria.Methods Hydrogen peroxide was prepared using a gas diffusion flow electrolysis cell.The reactive oxygen species were detected using electron spin resonance(EPR),and the disinfection performance on three microbial indicator bacteria,Escherichia coli,Staphylococcus aureus,and Bacillus subtilis black variety spores were observed as comparing to the commercially available hydrogen peroxide with same concentration.Results In addition to hydrogen peroxide,three metastable reactive oxygen species,namely superoxide anion,hydroxyl radical,and singlet oxygen existed in the electrocatalytic hydrogen peroxide disinfection solution,with a ratio of about 5:3:1.The content of the three new ecological reactive oxygen species could be maintained at a high level within about 1 h after the end of the electrocatalytic process.The electrocatalytic hydrogen peroxide disinfectant could meet the disinfection requirements of killing logarithms greater than 5.0 for three microbial indicator bacteria,including Escherichia coli,Staphylococcus aureus,and Bacillus subtilis black variant spores,after 1 min,5 min,and 30 min of disinfection.Conclusion The electrocatalytic production of hydrogen peroxide disinfectant can effectively improve the ability to kill microorganisms by generating hydrogen peroxide and generating various reactive oxygen species.
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