Observation on the bacteriostatic,antiviral effect and skin irritation of a potassium salt of ketoxime
Objective To observe the bacteriostatic and virucidal effects of potassium salt of 2,3-butanedione monoxime and test its skin irritation,so as to provide a basis for the practical application of potassium salt of ketoxime.Methods Broth dilution method,transmission electron microscopy observation,indirect immunofluorescence staining assay and animal testing methods were used to observe the bacteriostatic and viral inactivation effects and skin irritation of potassium salt of 2,3-butanedione monoxime.Results The minimum inhibitory concentration of 2,3-butanedione monoxime potassium salt on Staphylococcus aureus,Escherichia coli and Salmonella typhimurium was 12.5 mg/mL.The inhibition rate of the three test indicator bacteria was>99.9%after 10 min of action at a concentration of 100 mg/mL,and the damage of the cell membranes of Escherichia coli were obvious by the ketoxime potassium salt under the transmission electron microscopy observation.Encephalitis B virus or enterovirus 71 was negative for viral infection in cells by indirect immunofluorescence method after incubation with the ketoxime potassium salt.The ketoxime potassium salt had little irritation to the skin of white guinea pigs under the test concentration.Conclusion Potassium salt of 2,3-butanedione monoxime has strong killing effect on bacteria and viral and mild irritant to the intact skin of laboratory animals.
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