噬菌体Phi-X174和白色葡萄球菌气溶胶对紫外线消毒的抵抗力研究
Study on the resistance of phage Phi-X174 and Staphylococcus albus aerosol aerosols to ultraviolet light disinfection
张宝莹 1麻翠翠 2段弘扬 1王妍彦 1沈瑾1
作者信息
- 1. 中国疾病预防控制中心环境与健康相关产品安全所,北京 100021
- 2. 北京市疾病预防控制中心
- 折叠
摘要
目的 比较气溶胶状态下噬菌体Phi-X174和白色葡萄球菌对紫外线的抵抗力,探索噬菌体Phi-X174作为指示微生物用于空气病毒消毒效果评价的可能性.方法 检测一种紫外线消毒设备在不同消毒作用时间下对2种微生物的杀灭率,以D值为指标评价2种微生物气溶胶对紫外线的抵抗力大小.结果 以浓度为2×108 pfu/mL的噬菌体悬液雾化1.5 mL,空气消毒舱内噬菌体平均浓度为3.59×106 pfu/m3,组间误差率为8.13%;以浓度为8×106 cfu/mL的白色葡萄球菌菌液雾化2 mL,空气消毒舱内细菌平均浓度为7.10×105 cfu/m3,组间误差率为10.14%.紫外线消毒设备对噬菌体Phi-X174在5、10、15 min时的平均杀灭率为65.30%、86.89%、93.46%;对白色葡萄球菌在10、15、20 min时的平均杀灭率为74.87%、85.21%、94.92%.结论 噬菌体Phi-X174的D值在10～15 min,白色葡萄球菌的D值在15～20 min,即在气溶胶状态下,白色葡萄球菌对该紫外线消毒设备的抵抗力高于噬菌体Phi-X174.
Abstract
Objective To compare the resistance of phage Phi-X174 and Staphylococcus albus to ultraviolet light in aerosol state,and to explore the possibility of phage Phi-X174 as an indicator microorganism for the evaluation of the disinfection effect of air viruses.Methods The reproducibility of the killing rates of two microorganisms by a UV disinfection equipment at different disinfection times were tested,and the resistance of two types of microbial aerosols to UV light was evaluated using D value as the indicator.Results 1.5 mL of phage suspension with a concentration of 2×108 pfu/mL was atomized,and the average concentration of phage in the air disinfection chamber was 3.59×106 pfu/m3,with an inter-group error rate of 8.13%;2 mL of Staphylococcus albus suspension with a concentration of 8×106 cfu/mL was atomized,and the average concentration of bacteria in the air disinfection chamber was 7.10×105 cfu/m3,with an inter-group error rate of 10.14%.The average killing rates of phage Phi-X174 by ultraviolet disinfection equipment at 5,10,and 15 min were 65.30%,86.89%,and 93.46%,respectively.The average killing rates of Staphylococcus albus at 10,15,and 20 min were 74.87%,85.21%,and 94.92%,respectively.Conclusion The D value of phage Phi-X174 is between 10 and 15 min,and the D value of Staphylococcus albus is between 15 and 20 min.Staphylococcus albus has a higher resistance to the UV disinfection equipment than phage Phi-X174 in aerosol state.
关键词
噬菌体/白色葡萄球菌/气溶胶/紫外线/沉降率/抵抗力Key words
phage/Staphylococcus albus/aerosol/ultraviolet light/sedimentation rate/resistance引用本文复制引用
基金项目
首都卫生发展科研专项(首发2022-2G-4232)
出版年
2024
NETL
NSTL
万方数据