Study on the resistance of phage Phi-X174 and Staphylococcus albus aerosol aerosols to ultraviolet light disinfection
Objective To compare the resistance of phage Phi-X174 and Staphylococcus albus to ultraviolet light in aerosol state,and to explore the possibility of phage Phi-X174 as an indicator microorganism for the evaluation of the disinfection effect of air viruses.Methods The reproducibility of the killing rates of two microorganisms by a UV disinfection equipment at different disinfection times were tested,and the resistance of two types of microbial aerosols to UV light was evaluated using D value as the indicator.Results 1.5 mL of phage suspension with a concentration of 2×108 pfu/mL was atomized,and the average concentration of phage in the air disinfection chamber was 3.59×106 pfu/m3,with an inter-group error rate of 8.13%;2 mL of Staphylococcus albus suspension with a concentration of 8×106 cfu/mL was atomized,and the average concentration of bacteria in the air disinfection chamber was 7.10×105 cfu/m3,with an inter-group error rate of 10.14%.The average killing rates of phage Phi-X174 by ultraviolet disinfection equipment at 5,10,and 15 min were 65.30%,86.89%,and 93.46%,respectively.The average killing rates of Staphylococcus albus at 10,15,and 20 min were 74.87%,85.21%,and 94.92%,respectively.Conclusion The D value of phage Phi-X174 is between 10 and 15 min,and the D value of Staphylococcus albus is between 15 and 20 min.Staphylococcus albus has a higher resistance to the UV disinfection equipment than phage Phi-X174 in aerosol state.
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