Observation on the killing effect of common used disinfectants against three kinds of zoonotic pathogens
Objective To compare the killing effects of nine commonly used disinfectants on attenuated Mycobacterium bovis,Bacillus anthracis attenuated strains spores and Brucella spp attenuated strains.Methods The suspension qualitative bactericidal test was used to detect the effect of 84 disinfectant,0.5%iodophor,50%dibromohydantoin,30%hydrogen peroxide solution,75%ethanol,50%carbolic acid,0.2%chlorhexidine,3%benzalkonium bromide and 2%glutaraldehydee on three bacteria for 5 and 45 min,respectively.Bacterial culture method was used to evaluate the killing effect of each disinfectant.Results 0.55%84 disinfectant,0.5%iodophor,3%hydrogen peroxide solution,75%ethanol and 2%glutaraldehyde could effectively kill attenuated Mycobacterium bovis after 5 min,while 0.1%dibromohain and 0.3%benzalammonium bromide had no obvious killing effect even if time was extended to 45 min.Similarly,0.55%84 disinfectant solution,0.5%iodophor and 3%hydrogen peroxide solution had a certain efficacy on Bacillus anthracis attenuated strains spores only when incubating for a long time.75%ethanol,5%carbonic acid,0.2%chlorhexidine and 0.3%benzalammonium bromide could not completely kill Bacillus anthracis spores incubating for 45 min.Conclusions 75%ethanol and 0.55%84 disinfectant have good killing effect on attenuated Mycobacterium bovis and Brucella spp attenuated strains,but have no obvious killing effect on Bacillus anthracis attenuated strain spores.
disinfectantmycobacterium bovisBacillus anthracisbrucella sppsuspension qualitative bactericidal test
万方数据
维普
