Observation on the disinfection effect of a quaternary ammonium disinfectant at low temperature and its safety
Objective To observe the disinfection effect of a quaternary ammonium disinfectant with benzalkonlum chloride as the main ingredient at low temperature and its safety.Methods Carrier quantitative bactericdal test,simulated field disinfection test,acute oral toxicity of mice and micronucleus test were used to evaluate the disinfection effect and safety of a quaternary ammonium disinfectant at low temperature.Results The quaternary ammonium disinfectant stock solution contained benzalkonium chloride 3 500 mg/L.The the killing logarithms of Staphylococcus aureus and E.coli on plain cloth carrier were>5.00 when the liquid was soaked at-20℃for 4-12 min.In the simulated field test,the stainless steel disc bacterial carrier was placed on the vehicle at-20℃,and was disinfecized with the ammonium disinfectant solution spraying for 40 min.The killing rate of Staphylococcus aureus on the carrier was>99.9%.The LD50 value of the acute oral toxicity test of the quaternary ammonium disinfectant on mice was>5 000 mg/(kg·bw),and the micronucleus rate of polychromatic erythrocytes in the bone marrow of mice in the disinfectant treatment group had no statistical difference from that in the negative control group,but was significantly lower than that in the positive control group.Conclusion The bactericidal effect of the quaternary ammonium disinfectant at-20℃is good,and it belongs to the actual non-toxic grade.
low temperature disinfectantquaternary ammonium saltdisinfection effectsafety
万方数据
维普
