Inactivation effect of ozone and its combination with oxidants on African swine fever virus
Objective To investigate the inactivation effect of combined ozone and oxidants on African swine fever virus(ASFV),and to provide technical support for ASFV control strategies in livestock farming.Methods Inactivation effects of 20 mg/L ozone combined with different concentrations of KMnO4 and K2FeO4 on 1×107 HAD50/mL.ASFV were observed and compared with the inactivation achieved by ozone or oxidants alone.Virus inactivation rates were quantitatively analyzed by using fluorescence microscopy and ImageJ software.Results Using 20 mg/L ozone alone showed limited inactivation of high-concentration ASFV,achieving an inactivation rate of only 27.0%after 30 min.The combination of ozone with 5 mg/L KMnO4 resulted in an inactivation rate of 86.8%within 10 min.However,the inactivation rate of ozone combined with K2FeO4 was lower than that of K2FeO4 alone.Conclusion Ozone combined with KMnO4 has exhibited a synergistic effect,significantly enhancing the inactivation efficiency against ASFV.In contrast,no synergistic effect is observed with ozone combined with the alkaline oxidant K2FeO4,indicating that the acid-base properties should be carefully considered when selecting oxidants for combination with ozone.
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