Analysis of Factors Affecting the Inactivation of Cellulase and Its Application in Concentrated Laundry Detergent
The effects of different types of surfactants on cellulase inactivation were studied,a concentrated laundry detergent formula was designed with the optimal surfactant,and investigated the effect of concentrated laundry detergent pH on cellulase inactivation.The results indicated that anionic surfactants had a significant impact on the inactivation of cellulase,with the order of influence was LAS>K12>MES>AES>SNS-80.The effect of non-ionic surfactants on the inactivation of cellulase was the smallest,and the order of the effects of the four non-ionic surfactants on cellulase inactivation was as follows:APG0814>AEO-9≈AEO-7>SOE.The influence of zwitterionic surfactants was greater than that of non-ionic surfactants,but less than that of most anionic surfactants.Cationic surfactants could cause rapid deactivation and precipitation of cellulase.The concentrated laundry detergent with SNS-80,SEO,and AEO-9 as the main active ingredients maintained high cellulase activity under neutral conditions.After 4 weeks,the enzyme activity exceeded 55%.As the pH increased to above 11,the rate of enzyme inactivation increased.
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