Comparison of the Results of Suspension Method and Carrier Method for Testing the Bactericidal Effect of Disinfectants
Objective:To compare the bactericidal effect of disinfectants detected by suspension method and carrier method.Methods:The killing effect of three disinfectants on three typical bacteria in food was compared by suspension quantitative method(under clean condition and under condition with organic interference)and carrier quantitative method under the same conditions.Results:Under cleaning conditions,the concentration of 50 mg/L peroxyacetic acid,chlorine dioxide and quaternary ammonium salt for 2min,the killing value of Enterobacter sakazakii and Vibrio vulnificus were>5.00,The concentration of 100 mg/L peroxyacetic acid,chlorine dioxide and quaternary ammonium salt for 2min,and the killing value of Clostridium perfringens was>5.00;Under the condition of organic interference,the concentration of 100 mg/L peroxyacetic acid,chlorine dioxide and quaternary ammonium salt for 2min,the killing value of Enterobacter sakazakii was>5.00,the concentration of 50 mg/L peroxyacetic acid,chlorine dioxide and quaternary ammonium salt for 2min,the killing value of Vibrio vulnificus was>5.00,respectively.The concentration of 200 mg/L peroxyacetic acid,chlorine dioxide and quaternary ammonium salt for 1min,and the killing value of Clostridium perfringens was>5.00;Under the conditions of the carrier quantitative method,the concentration of 100 mg/L peroxyacetic acid,chlorine dioxide and quaternary ammonium salt for 2min,the killing value of Enterobacter sakazakii was>5.00,the concentration of 50 mg/L peroxyacetic acid,chlorine dioxide and quaternary ammonium salt for 1min,the killing value of Vibrio vulnificus was>5.00,respectively.The concentration of 200 mg/L peroxyacetic acid,chlorine dioxide and quaternary ammonium salt for 1min,and the killing value of Clostridium perfringens was>5.00.Conclusion:Different methods exhibit variations in the sterilization efficacy of disinfectants.The suspension quantification method has the best sterilization effect under cleaning conditions,followed by the carrier quantification method,and the suspension quantification method is the worst under the condition of containing mechanical interference substances.