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高效液相色谱法测定野菊花提取液中蒙花苷的含量

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采用高效液相二极管阵列检测法测定野菊花提取液中蒙花苷的含量.采用ZORBAX C18柱(4.6 mm×0.250 mm,5 μm),以0.02%醋酸水溶液和甲醇为流动相,流速0.8 mL/min,柱温35℃,检测波长334 nm,峰面积与浓度的线性关系良好(r>0.999 5);加标回收率95%~105%.该方法简单准确、快速高效,重现性好.
Determination of Linarin in Chrysanthemum Extract by HPLC-DAD
A method for determination of linarin in Chrysanthemum extract by HPLC-DAD was developed.A C18 column(4.6 mm×250 mm,5µm)was used with a mobile phase of methanol-0.02%acetic acid(49:51)and the flow rate was 0.8 mL/min.The wavelength for detection had been set at 334nm and the column temperature was 35℃.The recoverties of linarin were 95%-105%.This method gives accurate and reliable results,which is suitable for the quality control of Chrysanthemum extract.

inarinChrysanthemum extractHPLC-DAD

邬佩贤

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广州立白企业集团有限公司,广东广州,510170

野菊花提取液 蒙花苷 高效液相色谱

2024

中国洗涤用品工业
中国洗涤用品工业协会

中国洗涤用品工业

影响因子:0.105
ISSN:1672-2701
年,卷(期):2024.(9)