Determination of Triolein Content in Coicis Semen from Different Regions by UPLC-MS
OBJECTIVE To establish a ultra-high performance liquid chromatography with mass(UPLC-MS) method for determination of triolein content,and determine triolein content in Coicis Semen from 23 different regions.METHODS Using Waters Quattro Micro with mass,the column was Acquity UPLC BEH C18(2.1 mm × 100 mm,1.7 μm); the mobile phase was made up that A phase contained methanol,acetonitrile and 0.1％ formic acid solution with ammonia to pH 3 in the proportion for 19 ∶ 19 ∶ 2,and B phase was isopropyl alcohol,the proportion of A phase and B phase was 55 ∶ 45; column temperature was 45 ℃; the flow rate was 0.3 mL·min-1.Detection in ESI using positive ionization and multiple reactions monitoring mode(MRM) was applied to determine positive ions m/z 902.8→m/z 603.2.RESULTS The standard curve was Y=4.233 × 103X+9.172 × 102(r=0.999 5),the tirolein in linear range of 0.142-25.56 μg·mL-1 had a good linear relationship; the average recovery was 97.21％,RSD was 1.44％(n=9); and triolein content in Coicis Semen from different regions was different.CONCLUSION The established UPLC-MS method on determination of triolein content in Coicis Semen is sensitive,rapid,accurate,reproducible and can be used for the quality control of Coicis Semen.
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维普
