Determination of Cyperenone and α-Cyperone in Rat Plasma by UPLC-MS/MS and Their Pharmacokinetics
OBJECTIVE To establish an UPLC-MS/MS method for the determination of the concentrations of cyperenone andα-cyperone in rat plasma and to study the pharmacokinetics.METHODS Gradient elution was carried out on a Phenomennex C18(150 mm×2.0 mm,3 μm)column with acetonitrile-water as mobile phase.The column temperature was 30℃,injection volume was l μL,osthenite was used as the internal standard,electrospray ion source and positive ion mode were used.The m/z values of cyperenone,α-cyperone and osthenite were 219.1/135.1,219.1/111.0 and 245.0/123.0,respectively.The plasma concentrations of cyperenone and α-cyperone were measured,and the main pharmacokinetic parameters were calculated using DAS 2.0 software.RESULTS The linear relationship of cyperenone was good in the range of 10-500 ngmL-1(r=0.991 0),and the linear relationship of α-cyperone was good in the range of 2.5-300 ng·mL-1(r=0.994 1),RSDs of intra-day precision were less than 9.45％.RSDs of daytime precision were less than 9.09％.The recoveries were greater than 86.79％.After intragastric administration of essential oil extract(20 mgkg-1)from Cyperus rotundus L.in SD rats.The pharmacokinetic parameters of Cmax,AUC0-∞ and MRT(0-∞)of cyperenone and α-cyperone were(8 862.59±1 106.81)ng·L-1,(7 060.94±774.25)ng·L-1h,(3.21±0.72)h and(934.69±106.81)ng·L-1,(792.26±74.52)ng·L-1h,(4.94±0.82)h,respectively.CONCLUSION The established method can be used for the rapid and accurate determination of the concentration of cyperenone and α-cyperone in plasma,and can be used for the pharmacokinetic study of cyperenone and α-cyperone in rats in vivo.
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万方数据
维普
