Analysis of Chemical Constituents in Gleditsiae Fructus Abnormalis Based on UHPLC-Q-TOF-MS/MS
OBJECTIVE To Establish a liquid chromatography-mass spectrometry analysis method to comprehensively and quickly identify the chemical components in Gleditsiae Fructus Abnormalis.METHODS The ultra-high liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry(UHPLC-Q-TOF-MS/MS)was applied for the composition analysis of Gleditsiae Fructus Abnormalis extract;its mobile phase was 0.1％formic acid(A)-acetonitrile(B)with gradient elution at a flow rate of 0.3 mL·min-1.Accurate ion mass and secondary fragmentation information were obtained in negative ion mode,combined with databases and standard reference for structure elucidation.RESULTS A total of 78 compounds(32 flavonoids,15 phenolic acids,22 lignans and 9 chemical constituents of monoterpenoid acids)with 14 potential new compounds were screened.CONCLUSION This study comprehensively analyzes the chemical composition of Gleditsiae Fructus Abnormalis,and provides a basis for the investigation of the pharmacodynamic basis and quality standards of this drug.
NETL
NSTL
万方数据
维普
