Determination of Fourteen Fluorescent Whitening Agents in Cosmetics by HPLC-MS/MS
OBJECTIVE To establish a method for the determination of fourteen fluorescent whitening agents in cosmetics by HPLC-MS/MS.METHODS Samples were extracted on a Waters ACQUITY UPLC ®BEH C18(2.1 mm×50 mm,1.7 μm)column after ultrasonic extracted by DMF with the mobile phase consisted of methanol-0.1％ammonia water solution by gradient elution.The flow rate was 0.3 mL·min-1.The column temperature was 40 ℃.MS was performed using triple quadrupole mass spectrometry.Electrospray ionization source was operated in the positive/negative mode using multiple reaction monitoring scanning mode.RESULTS The results showed that there were good linear relationships for the fluorescent whitening agents in a certain concentration range with correlation coeffiicients(r)greater than 0.99.The limits of quantification were 0.01-20 μg·g 1 and the limits of detection were 0.004-8 μg·g-1.The average recoveries at three spiked levels were in the range of 85.4％-108.9％,and the relative standard deviation were in the range of 0.3％-7.2％.CONCLUSION The method has high sensitivity,strong specificity,simple and convenient operation,and is suitable for the detection of fourteen fluorescent whitening agents in cosmetics.
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万方数据
维普
