Simultaneous Determination of 8 Anti-infective Drugs in Anti-dandruff Hair Products by QuEChERS-liquid Chromatography Tandem Mass Spectrometry
OBJECTIVE To establish a QuECHERS-liquid chromatography-tandem mass spectrometry method for simultaneous determination of 3 triazole antifungal drugs including itraconazole,voriconazole,posaconazole,and 5 nitroimidazoles including ronidazole,dimetridazole,secnidazole,tinidazole and ornidazole.METHODS The sample was added into 1 mL of saturated sodium chloride solution and extracted with acetonitrile,and then purified by QuECHERS method.The separation was performed on a ZORBAX SB-C18 column.The mobile phase consisted of 0.1％formic acid acetonitrile-0.1％formic acid aqueous solution by gradient elution,electrospray ionization(ESI+)source was applied and operated in the positive mode.Multiple reaction monitoring mode was used to monitor ions.Matrix-matched calibration was used to quantitative calculation.RESULTS Eight anti-infective drugs showed good linear relationship in the range of 20.0-1 000.0 ngmL-1.The linear correlation coeffiicients of the method were＞0.995.At three levels,the average recoveries ranged from 90.3％to 113.0％,with the RSD(n=6)from 0.4％to 2.6％.The limits of quantification of the method were among 0.056-1.89 μg·g-1,and the limits of detection were among 0.014-0.56 μg·g-1.CONCLUSION This method is rapid,accurate and has good purification effect,which can be applied to the detection of 8 anti-infective drugs in anti-dandruff hair products.
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维普
