Stability of Mandelonitrile in Commonly Used Solvents and Its Determination in Jian'er Qingjie Mixture
OBJECTIVE To establish an HPLC method for investigating the stability of mandelonitrile in commonly used solvents and quantitation determination of mandelonitrile in Jian'er Qingjie mixture.METHODS The assay was performed on an Agilent TC-C18(250 mm×4.6 mm,5 μm)column with a mobile phase consisting of acetonitrile-0.1％phosphoric acid(23:27)pumped at a flow rate of 1.0 mL·min-1.The column temperature was 30 ℃ and the detection wavelength was set at 207 nm.The stability of the mandelonitrile solution prepared with solvents such as methanol,95％ethanol,acetonitrile,water,phosphoric acid solution with pH 2.0-6.0,and acetonitrile solution containing 1.0％glacial acetic acid was investigated using the peak reduction rate as the indicator.RESULTS Mandelonitrile was labile in methanol,95％ethanol or water and relatively stable in acetonitrile.The standard solutions of mandelonitrile prepared with phosphoric acid solutions at pH 2.0-3.5 or acetonitrile containing 1.0％glacial acetic acid were stable in 12 h.The linear range of mandelonitrile was 1.033-294.987 μg·mL-1(r=0.999 9)and the average recovery was 97.4％with RSD of 0.6％(n=9).The content range of mandelonitrile in 16 batches of Jian'er Qingjie mixture produced by 5 manufactures was 3.854-154.578 μg·mL-1.CONCLUSION The established method is simple and accurate.It can be used for the quality control of Jian'er Qingjie mixture.For almond aromatic water and its preparations,the influence of solvent on stability of mandelonitrile should be noticed.
万方数据
维普
