Explore the Mechanism of Steam-processed Polygonatum Sibiricum Polysaccharides in Prophylaxis and Treatment of Blood Deficiency Mice Model Based on Transcriptomics
OBJECTIVE To explore the mechanism of steam-processed Polygonatum sibiricum polysaccharides(SPSP)in prophylaxis and treatment of mice with blood deficiency syndrome(BDS)by RNA sequencing(RNA-seq)technology.METHODS The mice were randomly divided into five groups(10 mice in each group),namely normal group,model group,SPSP groups(0.1,0.4 g·kg-1),Danggui Buxue oral liquid(DOL)group.BDS model was induced in mice by acetylphenyl-hydrazine and cyclophosphamide.Blood routine,body weight and body temperature were tested after a consecutive administration for 14 d.The differential expressed genes(DEGs)related to anti-BDS by SPSP were screened through the transcriptome sequencing of the hepatic tissue in BDS mice.Functional annotation and enrichment analysis were performed to screen out the gene expression signaling pathways related to the treatment of SPSP on BDS mice.Quantitative polymerase chain reaction(qPCR)was used to verify the experiment.RESULTS Compared with the model group,SPSP(0.4 g·kg-1)could elevate the blood routine indexes such as red blood cell,white blood cell,hemoglobin,platelet,mean corpuscular hemoglobin concen-tration(P＜0.01),and reverse the body weight and body temperature to normal(P＜0.01 or P＜0.05).The result of transcriptomic analysis showed that the underlying mechanism was mainly related to hematopoietic cell line,retinol metabolism,steroid hormone biosynthesis,platelet activation,B cell receptor signaling pathway,and leukocyte transendothelial migration,etc.The result of qPCR showed that SPSP(0.4 g·kg-1)could elevate the expression of JAK1,STAT1 and GATA1 mRNA(P＜0.01 or P＜0.05).CONCLUSION SPSP has therapeutic effects on BDS.The key DEGs in the treatment of BDS by SPSP are mainly related to the restoration of hematopoietic function,regulation of hormone and immune function.The mechanism of SPSP on treatment of BDS might be the regulation of JAK1/STAT1 signaling pathway.
万方数据
维普
